Salivary Gland Development in Culture.
Salivary Gland Development in Culture.
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DOI：
10.1007/978-1-0716-1847-9_19
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发表时间：
2022-01-01
期刊：
影响因子：
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通讯作者：
Tucker, Abigail S
中科院分区：
文献类型：
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作者：
Gaete, Marcia;Teshima, Tathyane H N;Tucker, Abigail S
唾液腺是分支器官，通过芽和裂的形成形成一个表面积很大的器官。上皮细胞和间质之间来回传递信号来控制这一分支过程，副交感神经和环绕分支发育的血管提供额外的信号。这种分支形态发生可以在器官培养中成功重现，从而允许进入组织以跟踪发育并操纵组织相互作用和信号。为了培养腺体，过滤网格法已被广泛使用，可以将唾液腺作为一个完整的器官培养，或将腺体上皮分离培养，或在颅片中与周围的颅面组织一起培养。在这里，我们描述了每种方法的方法，并展示了培养各种物种腺体的适用性：小鼠，蛇和人类。从这些培养中得到的样本和数据可以用于形态学和分子分析，本章描述了一些例子，为我们对分支形态发生的理解带来了宝贵的知识。
Salivary glands are branching organs which develop by bud and cleft formation to create an organ with a large surface area. The epithelium and mesenchyme signal back and forth to control this branching process, with additional cues provided by the parasympathetic nerves and blood vessels that surround the developing branches. This branching morphogenesis can be recapitulated successfully in organ culture , allowing access to the tissue to follow development and manipulate the tissue interactions, and signals. To culture glands, the filter-grid method has been widely used, allowing the development of salivary glands cultured as a whole organ, or the gland epithelium in isolation, or with the surrounding craniofacial tissue in a cranial slice. Here, we describe the methods for each approach and show the applicability of culturing glands from a wide variety of species: mouse , snake, and human. The resulting samples and data from these cultures can be employed for morphological and molecular analysis, with some examples described in this chapter, bringing valuable knowledge to our understanding of branching morphogenesis.