Crosstalk between Nap1 protein and Cds1 checkpoint kinase to maintain chromatin integrity
Crosstalk between Nap1 protein and Cds1 checkpoint kinase to maintain chromatin integrity
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DOI：
10.1016/j.bbamcr.2008.03.019
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发表时间：
2008-09-01
影响因子：
5.1
通讯作者：
Aligue, Rosa
中科院分区：
文献类型：
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作者：
Grande, Maribel;Lambea, Eva;Aligue, Rosa
在酿酒酵母中，核小体组装蛋白Nap1参与多种细胞功能，如组蛋白穿梭进入细胞核、核小体组装、染色质重塑、转录控制和细胞周期调节。在Schizosaccharomyces pombe nap I中，零突变细胞是存活的，但它们表现出有丝分裂的延迟，这是由于缺乏复制Cds1 checkpoint激酶而得以挽救的。相反，缺乏dna损伤的Chk1检查点激酶无法挽救这种延迟。此外，双nap1 cds1突变细胞失去活力，细胞表现出正向的H2AX磷酸化，这表明nap1缺失细胞的活力是由于cds1激酶。我们还发现Nap1蛋白的过表达阻断了G1期的细胞周期。(c) 2008 Elsevier B.V.版权所有
The nucleosome assembly protein Nap1 has been implicated in various cellular functions such as histone shuttling into the nucleus, nucleosome assembly, chromatin remodelling, transcriptional control and cell-cycle regulation in Saccharomyces cerevisiae. In Schizosaccharomyces pombe nap I null mutant cells are viable but they showed a delay in the onset of mitosis which is rescued by the absence of the replication Cds1 checkpoint kinase. In contrast, the absence of the DNA-damage Chk1 checkpoint kinase is unable to rescue the delay. Moreover, the double nap1 cds1 mutant cells lose viability and cells show positive H2AX phosphorylation, suggesting that the viability of nap1-deleted cells is due to the Cds1 kinase. We also show that overexpression of Nap1 protein blocks the cell cycle in G1 phase. (c) 2008 Elsevier B.V. All rights reserved.