The Gold Nanorod-Biology Interface: From Proteins to Cells to Tissue.
The Gold Nanorod-Biology Interface: From Proteins to Cells to Tissue.
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DOI：
10.2174/1877946811303020002
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发表时间：
2013-04-01
期刊：
影响因子：
--
通讯作者：
Murphy, Catherine J
中科院分区：
文献类型：
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作者：
Boulos, Stefano P;Prigozhin, Maxim B;Murphy, Catherine J
金纳米棒强烈吸收和散射电磁波谱近红外部分的光，使其成为光学相干断层扫描 (OCT) 等成像技术的理想组织造影剂。纳米生物界面处会发生强烈的相互作用，例如蛋白质与金纳米棒结合形成“电晕”。为了实现纳米棒在造影剂等应用中的前景，我们必须更好地了解这些纳米材料与生物系统在分子、细胞和组织水平上的内在相互作用。在本文中，我们简要回顾了纳米棒-蛋白质界面。然后，我们提出了一些新的快速弛豫成像（FReI）测量方法，测量强吸收金纳米棒的存在如何影响蛋白质结合和折叠，同时考虑到内部过滤效应和纳米棒对荧光标记蛋白质的强猝灭效应。接下来，我们展示了金纳米棒的双光子光致发光可用于对组织结构中的纳米棒进行成像，使我们能够独立研究它们的组织分布，以便它们可以成功地用作光学相干显微镜中的造影剂。
Gold nanorods absorb and scatter light strongly in the near-infrared portion of the electromagnetic spectrum, making them ideal tissue contrast agents for imaging techniques such as optical coherence tomography (OCT). Strong interactions occur at the nano-bio interface, such as proteins binding to gold nanorods forming a 'corona.' To fulfill the promise of nanorods for applications such as contrast agents, we must better understand the intrinsic interactions of these nanomaterials with biological systems at the molecular, cellular and tissue level. In this paper, we briefly review the nanorod-protein interface. We then present some new fast relaxation imaging (FReI) measurements of how the presence of strongly-absorbing gold nanorods affects protein binding and folding, taking into account inner filter effects and the strong quenching effect of nanorods on fluorescent-labeled proteins. Next we show that two-photon photoluminescence of the gold nanorods can be used to image the nanorods in tissue constructs, allowing us to independently study their tissue distribution so they can be used successfully as contrast agents in optical coherence microscopy.