Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model.
Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model.
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在离体模型中采集患病人类外周动脉并测量内皮功能的技术。
DOI：
10.1016/j.jvs.2012.10.123
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发表时间：
2013
影响因子：
4.3
通讯作者：
Kashyap,VikramS
中科院分区：
文献类型：
--
作者：
Lakin,RyanO;Zhu,Weifei;Feiten,Lindsay;Kashyap,VikramS
已经在动物模型中研究了内皮功能障碍。然而，来自人类血管的内皮功能的直接证据是有限的。我们的目标是优化方法，在收获人类动脉截肢标本，确定内皮功能，并测量响应性tol-精氨酸，一氧化氮precurs.MethodsFresh截肢标本转移迅速从手术室的工作台实验室解剖和动脉收获的研究审查委员会批准的协议。在先导实验中检查了腘动脉和胫骨血管，导致在连续实验中使用胫前动脉。从14例截肢标本上切取下肢胫前动脉段。快速收集标本，并在组织浴装置中进行内皮依赖性舒张（EDR）研究、免疫组织化学和血管内超声衍生的虚拟组织学。共研究了47个环段。结果人类下肢动脉表现出低反应乙酰胆碱（EDR，24.9%;乙酰胆碱，10 - 4）。补充L-精氨酸可使EDR增加38.5%（P< .0001），N-硝基-L-精氨酸甲酯可使暴露于L-精氨酸的血管EDR消失（P< .0001）。所有血管的动脉反应性均完整（硝普钠非内皮依赖性舒张，113.2% ± 28.1%）。组织学和免疫组织化学证实了完整的内皮细胞形态分析，分化簇31，内皮型一氧化氮合酶，和β-内酰胺酶II染色。血管内超声衍生的虚拟组织学显示动脉粥样硬化负荷为11.9 ± 4.7 mm 3/cm，斑块分层显示纤维形态占主导地位（59.9%;坏死核心，16.9%;钙，11.2%）。斑块形态的变化与内皮功能或对L-arginine.ConclusionsHuman下肢动脉的反应性不相关，表明需要截肢的患者的基线内皮功能较低。在离体模型中通过补充L-精氨酸改善内皮功能障碍。这些结果支持增加人体血管中局部一氧化氮水平的策略。
ObjectiveEndothelial dysfunction has been studied in animal models. However, direct evidence of endothelial function from human vessels is limited. Our objectives were to optimize methods in harvesting human arteries from amputation specimens, determine endothelial function, and measure responsiveness tol-arginine, a nitric oxide precursor.MethodsFresh amputation specimens were transferred expeditiously from the operating room to the bench laboratory for dissection and arterial harvest in an Investigational Review Board-approved protocol. Popliteal and tibial vessels were examined in pilot experiments leading to the use of the anterior tibial artery in consecutive experiments. Human lower extremity anterior tibial artery segments were harvested from 14 amputation specimens. Specimens were rapidly collected and divided for endothelial-dependent relaxation (EDR) studies in a tissue bath apparatus, immunohistochemistry, and intravascular ultrasound-derived virtual histology. A total of 47 ring segments were studied. The data were compared with two-way analysis of variance.ResultsHuman lower extremity arteries exhibited low responsiveness to acetylcholine (EDR, 24.9%; acetylcholine, 10−4). L-arginine supplementation enhanced EDR by 38.5% (P< .0001).N-nitro-L-arginine methyl ester abrogated EDR (P< .0001) in vessels exposed to L-arginine. Arterial responsiveness was intact in all vessels (endothelial independent relaxation to sodium nitroprusside, 113.2% ± 28.1%). Histology and immunohistochemistry confirmed intact endothelium by morphometric analysis, cluster of differentiation 31, endothelial nitric oxide synthase, and arginase II staining. Intravascular ultrasound-derived virtual histology indicated atheroma burden was 11.9 ± 4.7 mm3/cm, and plaque stratification indicated fibrous morphology was predominant (59.9%; necrotic core, 16.9%; calcium, 11.2%). Variations in plaque morphology did not correlate with endothelial function or responsiveness to L-arginine.ConclusionsHuman lower extremity arteries demonstrate low baseline endothelial function in patients requiring amputation. Endothelial dysfunction is improved by L-arginine supplementation in an ex vivo model. These results support strategies to increase local levels of nitric oxide in human vessels.
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影响因子：
37.8
作者：
HODGSON, JM;MARSHALL, JJ
通讯作者：
MARSHALL, JJ
影响因子：
158.5
作者：
LUDMER, PL;SELWYN, AP;GANZ, P
通讯作者：
GANZ, P
勘误：肱动脉内皮依赖性血流介导的血管舒张的超声评估指南。
影响因子：
24
作者：
M. Corretti;T. Anderson;E. Benjamin;D. Celermajer;F. Charbonneau;M. Creager;J. Deanfield;H. Drexler;M. Gerhard;D. Herrington;P. Vallance;J. Vita;R. Vogel
通讯作者：
R. Vogel
急性动脉血栓形成导致内皮功能障碍：溶栓治疗的新范例。
影响因子：
4.3
作者：
V. Kashyap;T. Reil;W. Moore;T. Hoang;H. Gelabert;R. Byrns;L. Ignarro;J. Freischlag
通讯作者：
J. Freischlag
DOI：
10.1073/pnas.84.24.9265
发表时间：
1987-12-01
影响因子：
11.1
作者：
IGNARRO, LJ;BUGA, GM;CHAUDHURI, G
通讯作者：
CHAUDHURI, G