Quantitative trait loci affecting the 3D skull shape and size in mouse and prioritization of candidate genes in-silico.
Quantitative trait loci affecting the 3D skull shape and size in mouse and prioritization of candidate genes in-silico.
复制标题
DOI：
10.3389/fphys.2015.00092
复制
发表时间：
2015
影响因子：
4
通讯作者：
Cox TC
中科院分区：
文献类型：
--
作者：
Maga AM;Navarro N;Cunningham ML;Cox TC
We describe the first application of high-resolution 3D micro-computed tomography, together with 3D landmarks and geometric morphometrics, to map QTL responsible for variation in skull shape and size using a backcross between C57BL/6J and A/J inbred strains. Using 433 animals, 53 3D landmarks, and 882 SNPs from autosomes, we identified seven QTL responsible for the skull size (SCS.qtl) and 30 QTL responsible for the skull shape (SSH.qtl). Size, sex, and direction-of-cross were all significant factors and included in the analysis as covariates. All autosomes harbored at least one SSH.qtl, sometimes up to three. Effect sizes of SSH.qtl appeared to be small, rarely exceeding 1% of the overall shape variation. However, they account for significant amount of variation in some specific directions of the shape space. Many QTL have stronger effect on the neurocranium than expected from a random vector that will parcellate uniformly across the four cranial regions. On the contrary, most of QTL have an effect on the palate weaker than expected. Combined interval length of 30 SSH.qtl was about 315 MB and contained 2476 known protein coding genes. We used a bioinformatics approach to filter these candidate genes and identified 16 high-priority candidates that are likely to play a role in the craniofacial development and disorders. Thus, coupling the QTL mapping approach in model organisms with candidate gene enrichment approaches appears to be a feasible way to identify high-priority candidates genes related to the structure or tissue of interest.
登录
查看更多内容
影响因子：
3.8
作者：
HALEY, CS;KNOTT, SA
通讯作者：
KNOTT, SA
DOI：
10.1098/rspb.2007.1169
发表时间：
2008-01-07
影响因子：
4.7
作者：
Drake, Abby Grace;Klingenberg, Christian Peter
通讯作者：
Klingenberg, Christian Peter
影响因子：
2.4
作者：
Boell, Louis;Pallares, Luisa F.;Tautz, Diethard
通讯作者：
Tautz, Diethard
影响因子：
14.9
作者：
Chen J;Bardes EE;Aronow BJ;Jegga AG
通讯作者：
Jegga AG
DOI：
10.1002/dvdy.24170
发表时间：
2014-11
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Agrawal P;Wang M;Kim S;Lewis AE;Bush JO
通讯作者：
Bush JO