SYNCHRONIZATION OF PLASMODIUM-FALCIPARUM ERYTHROCYTIC STAGES IN CULTURE
SYNCHRONIZATION OF PLASMODIUM-FALCIPARUM ERYTHROCYTIC STAGES IN CULTURE
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DOI：
10.2307/3280287
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发表时间：
1979-01-01
影响因子：
1.3
通讯作者：
VANDERBERG, JP
中科院分区：
文献类型：
--
作者：
LAMBROS, C;VANDERBERG, JP
通过将培养的恶性疟原虫悬浮在5%D-山梨醇中，然后重新引入培养物中，实现了人恶性疟原虫红细胞期在培养中的同步发育。山梨醇治疗后立即，培养主要由单环状和多环状感染组成。同时，感染成熟期寄生虫的红细胞有不同程度的溶解。在山梨醇处理后48和96h，大约95%的寄生虫处于环状发育阶段，在24、72和120h也观察到较高比例的滋养体和裂殖体，D-甘露醇具有类似的选择性裂解作用。
Synchronous development of the erythrocytic stages of a human malaria parasite, P. falciparum, in culture was accomplished by suspending cultured parasites in 5% D-sorbitol and subsequent reintroduction into culture. Immediately after sorbitol treatment, cultures consisted mainly of single and multiple ring-form infections. At the same time, varying degrees of lysis of erythrocytes infected with the more mature stages of the parasite were evident. Approximately 95% of the parasites were in the ring stage of development at 48 and 96 h after sorbitol treatment; likewise, a high percentage of trophozoite and schizont stages was observed at 24, 72 and 120 h. D-mannitol produced similar, selective, lytic effects.