PRODUCTION OF MONOZYGOTIC (IDENTICAL) HORSE TWINS BY EMBRYO MICROMANIPULATION
PRODUCTION OF MONOZYGOTIC (IDENTICAL) HORSE TWINS BY EMBRYO MICROMANIPULATION
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DOI：
10.1530/jrf.0.0710607
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
PASHEN, RL
中科院分区：
文献类型：
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作者：
ALLEN, WR;PASHEN, RL
从23只小马驹的排卵后1-3天手术回收的2- 8-细胞胚胎（19）的卵裂球被机械分离，并以各种组合插入抽空的猪透明带中，制成27个半和17个四分之一显微操作胚胎。将其包埋在琼脂中，并在母羊结扎的输卵管中体内培养3.5-5天，以使其发育至桑椹胚晚期/囊胚早期。随后将13个半胚和17个四分之一胚手术或非手术移植到子宫，导致10例妊娠，包括2对单卵对。手术移植到最近没有用作胚胎供体的胚胎的成功率（10/20）高于手术或非手术移植到最近手术的胚胎（0/10）。
The blastomeres of 2- to 8-cell embryos (19) recovered surgically 1-3 days after ovulation from 23 pony mares were mechanically separated and inserted, in various combinations, into evacuated pig zonae pellucidae to make 27 half and 17 quarter micromanipulated embryos. These were embedded in agar and cultured in vivo in the ligated oviducts of ewes for 3.5-5 days to allow development to the late morula/early blastocyst stage. Subsequent surgical or non-surgical transfer of 13 half and 17 quarter embryos to mares resulted in 10 established pregnancies, including 2 monozygotic pairs. Surgical transfer to mares that had not been recently used as donors of embryos was more successful (10/20) than surgical or non-surgical transfer to recently operated mares (0/10).