In vivo visualisation of coronary artery development by high-resolution optical coherence tomography.
In vivo visualisation of coronary artery development by high-resolution optical coherence tomography.
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DOI：
10.1136/hrt.2007.120147
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发表时间：
2008-02-01
期刊：
影响因子：
--
通讯作者：
Yelbuz, T M
中科院分区：
文献类型：
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作者：
Norozi, K;Thrane, L;Yelbuz, T M
AO，升主动脉；LA，左心房；LV，左心室；PA，肺动脉；RA，右心房；RB，右头臂动脉起自升主动脉；RV，右心室。A中的箭头表示室间沟。箭头表示右冠状动脉(RCA)的走向；小箭头表示主动脉瓣尖端；星形箭头表示主动脉窦。BAR(B和C板)=100μm。BAR(D和E板)=600μm。心血管发育过程中最关键但知之甚少的过程之一是建立一个正常运作的冠状动脉(CA)系统。在此，我们介绍了在CA发育的三个关键阶段(第8天(视频1)、第9天(视频2、3和5)和第10天(视频4))从鸡胚胎心脏获得的发育CA的第一个活体记录。图像由高分辨率光学相干层析成像(OCT)生成。图A描绘的是9日龄雏鸡心脏的腹面图(与主动脉建立CA连接的阶段)，原始大小(上图为小图)，右侧放大倍数较大。虚线表示用于OCT扫描的矢状面(分别为B、D和E组)和组织学(C组)，以显示源自升主动脉的右冠状动脉(RCA)干。如图B所示，彩色多普勒的活体OCT记录显示首次出现舒张期右冠状动脉血流(黄色方框)，如图B所示。图C显示相应的组织学切片，图D显示第9天雏鸡心脏体积扫描数据的OCT图像。图E显示的是主动脉的三维重建，图D显示的是同一心脏的OCT容积扫描数据的RCA。
Ao, ascending aorta; LA, left atrium; LV, left ventricle; PA, pulmonary artery; RA, right atrium; RB, right brachiocephalic artery arising from the aorta ascendens; RV, right ventricle. Arrow in A shows the interventricular groove. Arrowheads indicate the course of the right coronary artery (RCA); small arrows, aortic valve cusps; star, aortic sinus. Bar (panels B and C)= 100 μm. Bar (panels D and E)= 600 μm.One of the most critical but poorly understood processes during cardiovascular development is the establishment of a functioning coronary artery (CA) system. We present here the first in vivo recordings of developing CAs obtained from the hearts of chick embryos at three critical stages of CA development (day 8 (video 1), day 9 (videos 2, 3 and 5) and day 10 (video 4)). Images were generated by high-resolution optical coherence tomography (OCT). Panel A depicts the ventral view of a day 9 chick heart (stage of established CA connection with the aorta) in original size (small image at top) and with higher magnification on the right. The dashed line indicates the sagittal section plane for OCT scanning (panels B, D and E, respectively) and histology (panel C) to visualise the right coronary artery (RCA) stem arising from the ascending aorta. In vivo OCT recording with colour Doppler demonstrates first onset of blood flow in the RCA in diastole (yellow box), as shown in panel B. Panel C depicts a corresponding histological section and panel D an OCT image from volume scan data in day 9 chick hearts. Panel E demonstrates a three-dimensional reconstruction of the aorta with the arising RCA of the OCT volume scan data of the same heart shown in panel D.