Multipotential mesenchymal stem cells from femoral bone marrow near the site of osteonecrosis
Multipotential mesenchymal stem cells from femoral bone marrow near the site of osteonecrosis
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DOI：
10.1634/stemcells.21-2-190
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发表时间：
2003-01-01
期刊：
影响因子：
5.2
通讯作者：
Jiang, CC
中科院分区：
文献类型：
--
作者：
Lee, HS;Huang, GT;Jiang, CC
基于干细胞的退行性疾病和损伤治疗在新时代很有希望。来自骨髓的多潜能间充质干细胞(MSCs)因其易于在培养中扩增并保持其多向分化潜能而处于领先地位。培养的MSCs的软骨和成骨能力的体外评估已经建立，这些实验中使用的BM完全来自健康供体，通过髂骨抽吸。目前尚不清楚从股骨获得的人骨髓是否也含有这些多潜能的MSCs。我们从两名接受全髋关节置换手术治疗股骨头坏死的患者的股骨近端采集骨髓，分离并培养扩增的MSCs至约20个群体倍增。这些细胞均呈Beta1整合素阳性。当细胞被制成聚集体，在含有转化生长因子-β3的培养液中培养14天后，细胞开始表达聚集素和II型胶原的mRNA，并在基质中沉积免疫反应性的H型胶原和硫酸蛋白多糖，这是软骨分化的标志。这些MSCs在体外也可以分化为成骨细胞系，表现为碱性磷酸酶活性的增强和矿物质含量在培养板上的沉积。这些结果表明，在髋关节手术中获得的股骨BM也含有多潜能的MSCs。这些数据表明，未来可能会使用来自原位骨髓的MSCs进行直接替代治疗，并可能通过使用这种方法获得的骨髓建立MSC库，从而绕过从健康捐赠者那里抽取髂骨骨髓的必要性。
Stem cell-based therapies for degenerative disorders and injuries are promising in the new era. Multipotential mesenchymal stem cells (MSCs) from bone marrow (BM) are on the leading edge because they are easy to expand in culture while maintaining their multilineage potential. In vitro assessment of the chondrogenic and osteogenic potentials of cultured MSCs has been established, and the BM used in those experiments was exclusively from healthy donors via iliac crest aspiration. It is unknown whether human marrow obtained from femurs also contains these multipotential MSCs. We collected marrow from proximal femurs of two patients undergoing total hip replacement surgery for femoral head osteonecrosis and isolated and culture expanded MSCs to about 20 population doublings. These cells were homogeneously positive for beta1-integrin. When pelleted into aggregates and cultured in a medium containing transforming growth factor-beta3 for 14 days, the cells began to express mRNA for aggrecan and collagen type II and to deposit immunoreactive collagen type H and sulfated proteoglycans in the matrix, hallmarks of chondrogenic differentiation. These MSCs could also be differentiated into osteocytic lineage in vitro, as shown by increased expression of alkaline phosphatase activity and deposition of mineral content onto culture plates. These results indicate that femoral BM obtained during hip surgeries also contained multipotential MSCs. These data imply that direct replacement therapy using MSCs from in situ marrow may be possible in the future and that an MSC bank may be established by using marrow from this approach, bypassing the necessity for iliac marrow aspiration from healthy donors.