Rate of reaction with nitric oxide determines the hypertensive effect of cell-free hemoglobin
Rate of reaction with nitric oxide determines the hypertensive effect of cell-free hemoglobin
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DOI：
10.1038/nbt0798-672
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发表时间：
1998-07-01
影响因子：
46.9
通讯作者：
Lemon, DD
中科院分区：
文献类型：
--
作者：
Doherty, DH;Doyle, MP;Lemon, DD
以血红蛋白为基础的细胞外氧载体的管理往往会导致血压轻度升高，为了测试是否一氧化氮（NO）清除是负责高血压的效果，我们构建和测试了一组重组血红蛋白，不同的反应率与NO。结果表明，快速反应的氧和脱氧血红蛋白与一氧化氮是高血压的根本原因。血压效应的大小与体外NO氧化速率直接相关。具有降低的NO-清除活性的血红蛋白可能比引起一氧化氮耗竭的血红蛋白更适合于某些治疗应用。
Administration of extracellular hemoglobin-based oxygen carriers often induces mild increases in blood pressure, In order to test whether nitric oxide (NO) scavenging is responsible for the hypertensive effect, we constructed and tested a set of recombinant hemoglobins that vary in rates of reaction with NO. The results suggest that the rapid reactions of oxy-and deoxyhemoglobin with nitric oxide are the fundamental cause of the hypertension. The magnitude of the blood-pressure effect correlates directly with the in vitro rate of NO oxidation. Hemoglobins with decreased NO-scavenging activity may be more suitable for certain therapeutic applications than those that cause depletion of nitric oxide.