Genome-Wide Host-Pathogen Interaction Unveiled by Transcriptomic Response of Diamondback Moth to Fungal Infection.
Genome-Wide Host-Pathogen Interaction Unveiled by Transcriptomic Response of Diamondback Moth to Fungal Infection.
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小菜蛾对真菌感染的转录组反应揭示了全基因组宿主-病原体相互作用
DOI：
10.1371/journal.pone.0152908
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Feng MG
中科院分区：
文献类型：
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作者：
Chu ZJ;Wang YJ;Ying SH;Wang XW;Feng MG
从全基因组角度了解害虫对白僵菌（真菌昆虫病原体）感染的反应对于真菌杀虫剂的遗传改良至关重要，但对此的探索很少。我们构建了感染处理 (hptI) 和对照处理 (hptC) 后 24、36 和 48 小时的小菜蛾幼虫的三对转录组，以深入了解基因组水平上的宿主-病原体相互作用。在24、36和48 hptI/hptC时分别有2143、3200和2967个宿主基因差异表达。这些感染反应基因（约占宿主基因组的15%）在各种免疫过程中富集，例如补体和凝血级联、蛋白质消化和吸收以及药物代谢-细胞色素P450。真菌渗透到角质层并开始发生宿主防御反应，在 24 hptI 时开始，随后在 36 hptI 时出现最强烈的宿主免疫反应，并在 48 hptI 时免疫力减弱。相比之下，44%的真菌基因在感染过程中差异表达，并在抗氧化活性、过氧化物酶活性和蛋白水解等多个生物过程中富集。在 24-48 hptI 期间共表达了 1636 个真菌基因，其中包括 116 个编码推定分泌蛋白的基因。我们的研究结果为昆虫与病原体相互作用提供了新的见解，并有助于探讨全球害虫真菌感染的分子机制。
Genome-wide insight into insect pest response to the infection of Beauveria bassiana (fungal insect pathogen) is critical for genetic improvement of fungal insecticides but has been poorly explored. We constructed three pairs of transcriptomes of Plutella xylostella larvae at 24, 36 and 48 hours post treatment of infection (hptI) and of control (hptC) for insight into the host-pathogen interaction at genomic level. There were 2143, 3200 and 2967 host genes differentially expressed at 24, 36 and 48 hptI/hptC respectively. These infection-responsive genes (~15% of the host genome) were enriched in various immune processes, such as complement and coagulation cascades, protein digestion and absorption, and drug metabolism-cytochrome P450. Fungal penetration into cuticle and host defense reaction began at 24 hptI, followed by most intensive host immune response at 36 hptI and attenuated immunity at 48 hptI. Contrastingly, 44% of fungal genes were differentially expressed in the infection course and enriched in several biological processes, such as antioxidant activity, peroxidase activity and proteolysis. There were 1636 fungal genes co-expressed during 24–48 hptI, including 116 encoding putative secretion proteins. Our results provide novel insights into the insect-pathogen interaction and help to probe molecular mechanisms involved in the fungal infection to the global pest.