Experimental trials with a thermostable Newcastle disease virus (strain I2) in commercial and village chickens in Tanzania
Experimental trials with a thermostable Newcastle disease virus (strain I2) in commercial and village chickens in Tanzania
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DOI：
10.1016/s0167-5877(99)00089-6
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发表时间：
2000-01-20
影响因子：
2.6
通讯作者：
Hyera, JMK
中科院分区：
文献类型：
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作者：
Wambura, PN;Kapaga, AM;Hyera, JMK
测定了接种I-2耐热新城疫（ND）疫苗的地方农村鸡和商品鸡的抗体应答和对毒力强的野地分离物攻击的保护水平。滴眼液接种村鸡的抗体应答显示，初次接种后30、60和90天，平均对数(2)滴度分别为6.1、5.4和3.6，而商品鸡的抗体应答在14、30和90天后分别为8.2、5.1和4.2。经饮水口服接种的村鸡，30天后的平均对数(2)滴度为3.4。在加强疫苗接种后，二次疫苗接种后30天和60天（即一次疫苗接种后60天和90天）的平均HI滴度分别为5.4和3.3。初次通过食物口服疫苗接种后30天记录的抗体应答平均对数(2)滴度为2.6；二次接种后30天和60天（即一次接种后60天和90天），平均对数(2)HI滴度分别为5.3和3.2。所有通过滴眼液接种的商业鸡和乡村鸡均在攻毒试验中存活，而通过饮用水和食物接种的乡村鸡在初次接种后30天的保护水平分别为80%和60%。而在加强接种后30天，保护水平为100%。在二次接种后60天，口服接种鸡的保护水平再次下降至80%。在攻毒试验中使用的所有对照鸡均出现临床ND，并在接种毒力病毒后3-5天死亡。实验结果表明，I-2株新城疫病毒似乎是无毒的，具有免疫原性，并且对新城疫病毒的强毒分离株具有高度的保护作用。它可能是一种适合用于农村鸡的疫苗，可使它们接种新城疫。(C) 2000 Elsevier Science B.V.版权所有
Antibody responses in indigenous village and commercial chickens vaccinated with I-2 thermostable Newcastle disease (ND) vaccine and protection levels against challenge with a virulent field isolate were determined. The antibody response of village chickens vaccinated by eye drop revealed that 30, 60 and 90 days after primary vaccination, the mean log(2) HI titres were 6.1, 5.4 and 3.6, respectively, whereas for commercial chickens, the antibody response after 14, 30 and 90 days were 8.2, 5.1 and 4.2, respectively. Village chickens vaccinated orally via drinking water had mean log(2) HI titres of 3.4 after 30 days. After booster vaccination, the mean HI titre was 5.4 and 3.3 after 30 and 60 days post-secondary vaccination (i.e. 60 and 90 days after primary vaccination). Antibody response of mean log(2) HI titres of 2.6 was recorded 30 days after primary vaccination orally through food; 30 and 60 days after secondary vaccination (i.e. 60 and 90 days after primary vaccination), mean log(2) HI titres were 5.3 and 3.2, respectively. All commercial and village chickens vaccinated by eye drop survived the challenge trial whereas village chickens vaccinated through drinking water and food had protection levels of 80% and 60% 30 days after primary vaccination, respectively. However, 30 days after booster vaccination, the protection level was 100%. At 60 days after secondary vaccination, the protection level dropped again to 80% for chickens vaccinated orally. All control chickens used in the challenge trials developed clinical ND and died 3-5 days after inoculation with the virulent virus. Supported by laboratory findings, I-2 strain of NDV seemed to be avirulent, immunogenic and highly protective against Virulent isolates of NDV. It may be a suitable vaccine to use in village chickens to vaccinate them against ND in rural areas. (C) 2000 Elsevier Science B.V. All rights reserved.