Targeted expression of bgl23-D, a dominant-negative allele of ATCSLD5, affects cytokinesis of guard mother cells and exine formation of pollen in Arabidopsis thaliana
Targeted expression of bgl23-D, a dominant-negative allele of ATCSLD5, affects cytokinesis of guard mother cells and exine formation of pollen in Arabidopsis thaliana
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DOI：
10.1007/s00425-023-04097-0
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发表时间：
2023-02
期刊：
影响因子：
4.3
通讯作者：
Md. Firose Hossain;A. Dutta;Takamasa Suzuki;T. Higashiyama;Chiharu Miyamoto;S. Ishiguro;Takanori Maruta;Yuki Muto;K. Nishimura;Hideki Ishida;Mostafa Aboulela;Takushi Hachiya;T. Nakagawa
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文献类型：
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作者：
Md. Firose Hossain;A. Dutta;Takamasa Suzuki;T. Higashiyama;Chiharu Miyamoto;S. Ishiguro;Takanori Maruta;Yuki Muto;K. Nishimura;Hideki Ishida;Mostafa Aboulela;Takushi Hachiya;T. Nakagawa
结论靶向表达atcsld5的显性阴性等位基因bgl23- d，是分析atcsld5在植物特定细胞和组织中功能的一种有效的遗传方法。摘要气孔是植物体内气体和水分交换的关键细胞结构，其发育受多种基因的影响。我们找到了theA。百吉饼23- d （bgl23-D）突变体显示异常的百吉饼状单保卫细胞。bgl23- d是theA中一个新的显性突变。据报道，该基因在保护母细胞的分裂中起作用。bgl23- d的显性性状被用来抑制ATCSLD5在特定细胞和组织中的功能。TransgenicA。表达bgl23- dcdna的蓝藻具有气孔谱系基因SDD1、MUTE和fama的启动子，在bgl23- d突变体中观察到百吉饼状气孔。特别是fama启动子表现出更高频率的百吉饼形气孔和严重的细胞分裂缺陷。在带sp11启动子的绒毡层或带atsp146启动子的花药中，bgl23- dcdna的表达会导致外叶模式和花粉形状的缺陷，这些新表型在bgl23- d突变体中没有表现出来。这些结果表明，bgl23-D抑制了未知的ATCSLD(s)在绒毡层中发挥外膜形成功能。此外,transgenicA。带有sdd1、MUTE和fama启动子的表达gl23- dcdna的塔兰结花序直径增大，叶片生长加快。综上所述，这些发现表明bgl23- dmutation可能是一个有用的遗传工具，用于分析ATCSLDs的功能和操纵植物生长。
Main conclusionTargeted expression ofbgl23-D, a dominant-negative allele ofATCSLD5, is a useful genetic approach for functional analysis of ATCSLDs in specific cells and tissues in plants.AbstractStomata are key cellular structures for gas and water exchange in plants and their development is influenced by several genes. We found theA. thaliana bagel23-D(bgl23-D) mutant showing abnormal bagel-shaped single guard cells. Thebgl23-Dwas a novel dominant mutation in theA. thalianacellulose synthase-like D5(ATCSLD5) gene that was reported to function in the division of guard mother cells. The dominant character ofbgl23-Dwas used to inhibit ATCSLD5 function in specific cells and tissues. TransgenicA. thalianaexpressingbgl23-DcDNA with the promoter of stomata lineage genes,SDD1,MUTE, andFAMA, showed bagel-shaped stomata as observed in thebgl23-Dmutant. Especially, theFAMApromoter exhibited a higher frequency of bagel-shaped stomata with severe cytokinesis defects. Expression ofbgl23-DcDNA in the tapetum withSP11promoter or in the anther withATSP146promoter induced defects in exine pattern and pollen shape, novel phenotypes that were not shown in thebgl23-Dmutant. These results indicated that bgl23-D inhibited unknown ATCSLD(s) that exert the function of exine formation in the tapetum. Furthermore, transgenicA. thalianaexpressingbgl23-DcDNA withSDD1,MUTE, andFAMApromoters showed enhanced rosette diameter and increased leaf growth. Taken together, these findings suggest that thebgl23-Dmutation could be a helpful genetic tool for functional analysis of ATCSLDs and manipulating plant growth.