Synthesis and evaluation of fosfomycin group end-capped packing materials for hydrophilic interaction liquid chromatography
Synthesis and evaluation of fosfomycin group end-capped packing materials for hydrophilic interaction liquid chromatography
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磷霉素基团封端亲水作用液相色谱填料的合成及评价
DOI：
10.1016/j.chroma.2021.462529
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发表时间：
2021
影响因子：
4.1
通讯作者：
Chen Lingxin
中科院分区：
文献类型：
--
作者：
Song Zhihua;Li Jinhua;Lu Wenhui;Li Bowei;Liu Jinqiu;Wang Yaqi;Wang Yumeng;Zhang Zhong;Chen Lingxin
亲水相互作用液相色谱法（HILIC）在分析高极性化合物中起着重要作用。本研究选择磷霉素(F)作为新型封端试剂，因为其环氧基易于水解。制备了具有良好化学和物理稳定性的氧化锆包覆二氧化硅（ZrO2/SiO2）材料。将d -葡萄糖-6-磷酸(G)和d -果糖- 1,6-二磷酸（FDP）分别修饰在ZrO2/ sio2微球的内外表面。然后通过刘易斯酸碱相互作用将新的封端试剂(F)键合到改性材料的表面。表征了固定相（SPs）的形貌、Zr含量、孔径、孔体积和碳含量。最后，将得到的端盖SPs用于分离生物碱和苯甲酸。多种相互作用，包括HILIC，静电排斥，离子交换和氢键，有助于分析物在SPs上的保留。在F-FDP-ZrO2/ sio2柱上，小檗碱的理论板数为31,700板/m，不对称系数为1.63，具有良好的色谱性能。FDP-ZrO2/ sio2色谱柱具有良好的酸碱稳定性，可用于雪碧和人参皂苷中苯甲酸的分离分析。因此，结果表明使用F作为末端封盖试剂具有很大的潜力。
Hydrophilic interaction liquid chromatography (HILIC) plays an important role in the analysis of compounds having high polarity. In this study, fosfomycin (F) was chosen as a new end-capping reagent, owing to the facile hydrolysis reaction of its epoxy group. Zirconia coated silica (ZrO2/SiO2) materials having good chemical and physical stability were prepared. D-glucose-6-phosphate (G) and D-fructose1,6-bisphosphate (FDP) were modified onto the inner and outer surfaces of the ZrO2/SiO2microbeads. The new end-capping reagent (F) was then bonded onto the surface of the modified material through Lewis acid-base interactions. The properties (morphology, Zr content, pore size, pore volume, and carbon content) of the stationary phases (SPs) were characterized. Finally, the resulting end-capped SPs were employed to separate alkaloids and benzoic acids. Multiple interactions, including HILIC, electrostatic repulsion, ion exchange and hydrogen bonding, contributed to the retention of the analytes on the SPs. On the F-FDP-ZrO2/SiO2column, a theoretical plate number of 31,700 plates/m and an asymmetry factor of 1.63 were achieved for berberine, exhibiting good chromatographic performance. Furthermore, the FDP-ZrO2/SiO2column showed good acid-base stability and good potential for the analysis of benzoic acid in Sprite and ginsenoside separations. Thus, the results indicated the significant potential of using F as an end-capping reagent.