Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields
Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields
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生理脉冲直流电场诱导角质形成细胞趋电性
DOI：
10.1016/j.bioelechem.2019.02.001
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发表时间：
2019-06-01
影响因子：
5
通讯作者：
Zhang, Jiaping
中科院分区：
文献类型：
--
作者：
Ren, Xi;Sun, Huanbo;Zhang, Jiaping
内源性电场(EFS)指导皮肤创面角质形成细胞的迁移(趋电性)，外源性EFS可以促进创面愈合，但其潜在益处受到恒定直流电流(DC)通过组织的副作用的限制。相比之下，对于脉冲直流(以间歇输出为特征)，可以调整参数以将电流的不利影响降至最低。然而，目前尚不清楚脉冲DC是否能可靠地诱导角质形成细胞的趋电性，在本研究中，在电趋化室中使用原代角质形成细胞，我们发现生理强度的脉冲DCEF(EF=150 mV/mm，占空比=60%，频率=0.1 Hz)可以诱导强烈的趋电性。这种效应依赖于电压和占空比，但与频率无关。正如预测的那样，脉冲DCEF比恒定DCEF检测到更少的电化学反应和细胞毒性反应。综上所述，我们在这里首次证明，脉冲DCEF可以触发角质形成细胞的趋电性，与恒定DCEF诱导的趋电性相当，同时将电化学副作用降至最低。这些发现支持了脉冲DCEF设备的未来发展，以改善人类患者的伤口愈合。(C)《2019年》，爱思唯尔出版。
Endogenous electric fields (EFs) direct the migration (electrotaxis) of keratinocytes in skin wounds, and the exogenous application of EFs may therefore improve wound healing, but the potential benefits are limited by the side effects of constant direct current (DC) passing through tissues. In contrast, with pulsed DC (characterized by intermittent output), parameters can be adjusted to minimize the adverse effects of electric currents. However, it remains unknown whether pulsed DC can reliably induce keratinocyte electrotaxis, In this study, using primary keratinocytes in an electrotaxis chamber, we found that a pulsed DCEF at physiological strength (EF = 150 mV/mm, duty cycle = 60%, frequency = 0.1 Hz) could induce robust electrotaxis. This effect was dependent on both voltage and duty cycle, but not on frequency. As predicted, fewer electrochemical reactions and cytotoxic reactions were detected with pulsed DCEF than with constant DCEF. In summary, we here demonstrate for the first time, that pulsed DCEF can trigger keratinocyte electrotaxis comparable to that induced by constant DCEF, while minimizing the electrochemical side effects. These findings support the future development of a pulsed DCEF device to improve wound healing in human patients. (C) 2019 Published by Elsevier B.V.