Mapping of antibody specificities to VH gene families.
Mapping of antibody specificities to VH gene families.
复制标题
VH 基因家族的抗体特异性图谱。
DOI：
10.1007/bf00352838
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发表时间：
1989
期刊：
影响因子：
3.2
通讯作者：
Schulze,DH
中科院分区：
文献类型：
--
作者：
Kelsoe,G;Miceli,R;Cerny,J;Schulze,DH
VH基因片段代表原基VH基因元件重复复制和随后多样化的产物。人们普遍认为，通过病原体进行的自然选择在这一过程中发挥了主导作用。在这里，我们筛选了一些3.7 × 104个C μ+的有丝分裂原激活的B细胞集落，用于产生对磷酸胆碱或鸡蛋溶菌酶特异性的抗体以及VHX-24、S107、Q52或J558基因家族的表达。这些基因家族的表达频率与其基因组的复杂性成正比，无论是在大肠杆菌和抗原特异性Cμ+菌落。因此，编码等同抗体结合位点的能力均匀地分散在HBV家族中。这一结果表明，单个VH基因还没有进化到解决特定的抗原。
VHgene segments represent the products of the repeated duplication and subsequent diversification of a primordialVgene element. It is widely assumed that natural selection, operating via pathogens, has played the dominant role in this process. Here, we screen some 3.7 × 104Cμ+colonies of mitogen-activated B cells for the production of antibodies specific for phosphorylcholine or hen egg lysozyme and expression of theVHX-24, S107, Q52, orJ558gene families. These gene families were expressed at frequencies proportional to their genomic complexity among both unselected and antigen-specific Cμ+colonies. Thus, the capacity to encode equivalent antibody-combining sites is dispersed uniformly amongVHfamilies. This result suggests that individualVHgenes have not evolved to address specific antigens.