The application of patch clamp methods to ocular epithelia.
The application of patch clamp methods to ocular epithelia.
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膜片钳方法在眼上皮细胞中的应用。
DOI：
10.3109/02713688509025155
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发表时间：
1985
影响因子：
2
通讯作者：
J. Rae
中科院分区：
文献类型：
--
作者：
J. Rae
膜片电压箝位技术和全细胞箝位技术已应用于多种动物的晶状体和角膜。晶状体上皮细胞的基膜和顶膜、晶状体纤维的前后表面、角膜内皮细胞的顶膜和第二层角膜上皮细胞的顶膜中都发现了大量的离子通道。到目前为止，在深晶状体纤维膜中还没有发现离子通道。眼上皮膜内存在9 ~ 11种不同类型的钾离子通道，几种不同类型的非选择性阳离子通道，以及一种单位电导较大的非选择性阳离子通道。钠离子选择性通道很少被发现，而氯离子选择性通道则完全未被发现。有几个渠道尚未明确确定。使用千兆姆密封技术，可以显示青蛙晶状体上皮细胞膜以钾通道为主。此外，还描述了一种技术，用于使用25- 30ps非选择性阳离子通道的逆转电位来测量上皮细胞的静息电压而不穿透它们。晶状体离子通道定位研究的结果与先前使用全晶状体阻抗和离子替代技术的晶状体通道定位研究仅在定性上一致。提出了使用膜片钳进行离子通道定位的局限性。
The techniques of patch voltage clamping and whole cell clamping have been applied to the lenses and corneas of several species of animals. Numerous ion channels have been found in the basal and apical membranes of lens epithelial cells, anterior and posterior surface lens fibers, apical membrane of corneal endothelial cells, and apical membrane of the second layer of corneal epithelial cells. No ion channels have been found in deep lens fiber membranes to date. There are 9-11 different kinds of potassium channels in ocular epithelial membranes, several different kinds of non-selective cation channels, and one non-selective channel with a large unit conductance. Sodium selective channels are seen only rarely while chloride selective channels have not been seen at all. Several channels have not yet been identified unequivocally. Using the gigohm seal technique, it is possible to show that the frog lens epithelial cell membrane is dominated by potassium channels. Also, a technique is described for using the reversal potential of a 25-30 pS non-selective cation channel to measure the resting voltage of epithelial cells without penetrating them. The results of lens ion channel localization studies are in only qualitative agreement with previous lens channel localization studies which used whole lens impedance and ion substitution techniques. Limitations of using the patch clamp for ion channel localization are presented.
来自切除的肌膜斑块的单通道电流。
DOI：
10.1073/pnas.77.11.6930
发表时间：
1980
影响因子：
11.1
作者：
Horn,R;Patlak,J
通讯作者：
Patlak,J