Novel alginate-stabilized doxorubicin-loaded nanodroplets for ultrasounic theranosis of breast cancer
Novel alginate-stabilized doxorubicin-loaded nanodroplets for ultrasounic theranosis of breast cancer
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DOI：
10.1016/j.ijbiomac.2016.09.008
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发表时间：
2016-12-01
影响因子：
8.2
通讯作者：
Mokhtari-Dizaji, Manijhe
中科院分区：
文献类型：
--
作者：
Baghbani, Fatemeh;Mortarzadeh, Fathollah;Mokhtari-Dizaji, Manijhe
全氟碳纳米乳剂是一类新型的多功能刺激响应性纳米载体，其联合收割机结合了被动靶向药物载体、超声成像造影剂和超声响应性药物递送系统的性质。采用纳米乳液法制备了海藻酸钠稳定的全氟己烷（PFH）纳米微滴，并对其超声响应性、成像和治疗性能进行了研究。阿霉素负载到纳米液滴（39.2nm）中，包封率为92.2%。阿霉素纳米液滴的体外释药过程为明显的双相释放过程，在pH约为7.4的PBS中孵育24 h后，阿霉素纳米液滴的释药率为12.6%。用28 kHz治疗超声超声处理10 min触发PFH纳米液滴中的液滴到气泡转变，这导致85.95%的阿霉素从纳米液滴中释放。在乳腺癌小鼠模型中，超声介导的多柔比星负载PFH纳米液滴治疗显示出优异的抗癌效果，其特征在于肿瘤消退。对于其中多柔比星加载的纳米液滴与超声组合的组观察到完全肿瘤消退，而多柔比星加载的纳米液滴的肿瘤生长抑制为89.6%。这些多功能的纳米液滴，具有良好的治疗和超声性能，可能是有前途的药物输送系统的化疗应用。(C)2016爱思唯尔B.V.保留所有权利。
Perfluorocarbon nanoemulsions are a new class of multifunctional stimuli-responsive nanocarriers which combine the properties of passive-targeted drug carriers, ultrasound imaging contrast agents, and ultrasound-responsive drug delivery systems. Doxorubicin-loaded alginate stabilized perflourohexane (PFH) nanodroplets were synthesized via nanoemulsion preparation method and their ultrasound responsivity, imaging, and therapeutic properties were studied. Doxorubicin was loaded into the nanodroplets (39.2 nm) with encapsulation efficiency of 92.2%. In vitro release profile of doxorubicin from nanodroplets was an apparently biphasic release process and 12.6% of drug released from nanodroplets after 24h incubation in PBS, pH approximate to 7.4. Sonication with 28 kHz therapeutic ultrasound for 10 min triggered droplet-to-bubble transition in PFH nanodroplets which resulted in the release of 85.95% of doxorubicin from nanodroplets. Microbubbles formed by acoustic vaporization of the nanodroplets underwent inertial cavitation.In the breast cancer mice models, ultrasound-mediated therapy with doxorubicin-loaded PFH nanodroplets showed excellent anti-cancer effects characterized by tumor regression. Complete tumor regression was observed for the group in which doxorubicin-loaded nanodroplets were combined with ultrasound, whereas the tumor growth inhibition of doxorubicin loaded nanodroplets was 89.6%. These multifunctional nanodroplets, with excellent therapeutic and ultrasound properties, could be promising drug delivery systems for chemotherapeutic application. (C) 2016 Elsevier B.V. All rights reserved.