Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation
Pentatricopeptide repeat domain protein 3 associates with the mitochondrial small ribosomal subunit and regulates translation
复制标题
DOI：
10.1016/j.febslet.2009.04.048
复制
发表时间：
2009-06-18
期刊：
影响因子：
3.5
通讯作者：
Filipovska, Aleksandra
中科院分区：
文献类型：
--
作者：
Davies, Stefan M. K.;Rackham, Oliver;Filipovska, Aleksandra
哺乳动物线粒体转录的基本成分和机制已经被描述，然而，参与mRNA加工、翻译和稳定性的成分在很大程度上仍然未知。在植物中，五肽结构域rna结合蛋白调节线粒体转录本的稳定性、表达和翻译。在这里，我们研究了一种未被鉴定的哺乳动物五肽结构域蛋白，五肽重复结构域蛋白3 （pentatricopeptide repeat domain protein 3, PTCD3）的作用，并表明它是一种与线粒体核糖体小亚基相关的线粒体蛋白。PTCD3敲低和过表达不影响线粒体mRNA水平，提示PTCD3不参与RNA加工和稳定性。然而，在143B骨肉瘤细胞中，降低PTCD3可降低线粒体蛋白合成、线粒体呼吸以及复合物III和IV的活性，提示PTCD3在线粒体翻译中起作用。结构化摘要：MINT-7033995: PTCD3（单蛋白：Q96EY7）通过串联亲和纯化（MI: 0676）与MRPS15（单蛋白：P82914）结合（MI: 0914） (C) 2009年欧洲生化学会联合会。Elsevier B.V.版权所有。
The basic components and mechanisms of mitochondrial transcription in mammals have been described, however, the components involved in mRNA processing, translation and stability remain largely unknown. In plants, pentatricopeptide domain RNA-binding proteins regulate the stability, expression and translation of mitochondrial transcripts. Here, we investigated the role of an uncharacterized mammalian pentatricopeptide domain protein, pentatricopeptide repeat domain protein 3 (PTCD3), and showed that it is a mitochondrial protein that associates with the small subunit of mitochondrial ribosomes. PTCD3 knockdown and over expression did not affect mitochondrial mRNA levels, suggesting that PTCD3 is not involved in RNA processing and stability. However, lowering PTCD3 in 143B osteosarcoma cells decreased mitochondrial protein synthesis, mitochondrial respiration and the activity of Complexes III and IV, suggesting that PTCD3 has a role in mitochondrial translation.Structured summary: MINT-7033995: PTCD3 (uniprotkb: Q96EY7) associates (MI: 0914) with MRPS15 (uniprotkb: P82914) by tandem affinity purification (MI: 0676) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.