Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes.
Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes.
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大鼠树突状细胞充当辅助细胞，控制 T 淋巴细胞有丝分裂反应所需的可溶性因子的产生。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
W. Bowers
中科院分区：
文献类型：
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作者：
W. Klinkert;J. Labadie;J. O'Brien;C. Beyer;W. Bowers
用高碘酸盐或神经氨酸酶加半乳糖氧化酶处理诱导的T淋巴细胞转化需要辅助细胞的参与。开发了用于大鼠淋巴结细胞分级分离的程序，通过该程序，大部分淋巴细胞可以作为不响应促有丝分裂刺激的细胞的主要群体被回收，除非添加来自分离的次要群体的辅助细胞。进一步纯化使每个细胞的辅助活性总体增加了1000倍，产率为50-70%。最纯的制备物几乎不含巨噬细胞，并且含有超过90%的典型树突状细胞。最大的反应发生在只有一个树突状细胞每200高碘酸盐处理的淋巴细胞的比例。因此，这一证据有力地表明，在大鼠中，树突状细胞-而不是巨噬细胞-起辅助细胞的作用。此外，树突状细胞的数量在一个准备支配的有丝分裂反应的大小，并限制在未分级淋巴结细胞的情况下。此外，当用高碘酸盐或用神经氨酸酶加半乳糖氧化酶氧化时，树突状细胞充当未经处理的应答淋巴细胞的非常有效的间接刺激物。树突状细胞的两种功能似乎都缺乏种属特异性，因为小鼠树突状细胞在与大鼠应答淋巴细胞一起测试时非常活跃。一种可溶性因子（辅助细胞替代因子），由淋巴结或脾细胞经氧化有丝分裂培养产生，使原本无反应的有丝分裂原处理的淋巴细胞产生反应。树突状细胞是产生这种因子所必需的，但可能不是其产生的唯一原因。
Transformation of T lymphocytes, induced by treatment with periodate or with neuraminidase plus galactose oxidase, requires the participation of accessory cells. Procedures were developed for the fractionation of rat lymph node cells, by which most of the lymphocytes can be recovered as a major population of cells that do not respond to mitogenic stimulation unless accessory cells from a separated minor population are added. Further purification led to a 1000-fold overall increase in accessory activity per cell, with a 50-70% yield. The purest preparations were virtually free of macrophages and contained more than 90% typical dendritic cells. Maximum responses occurred at a ratio of only one dendritic cell per 200 periodate-treated lymphocytes. This evidence thus indicates strongly that in rats, dendritic cells--not macrophages--function as accessory cells. Further, the number of dendritic cells in a preparation governed the magnitude of the mitogenic response and was limiting in the case of unfractionated lymph node cells. In addition, when oxidized with periodate or with neuraminidase plus galactose oxidase, the dendritic cell served as a very potent indirect stimulator of untreated responder lymphocytes. Both functions of the dendritic cell appeared to lack species specificity, since mouse dendritic cells were very active when tested with rat responder lymphocytes. A soluble factor (accessory cell-replacing factor), produced by cultures of lymph node or spleen cells subjected to oxidative mitogenesis, enabled otherwise unresponsive mitogen-treated lymphocytes to respond. Dendritic cells were required for the production of this factor but may not be solely responsible for its production.