Subdiffusion of proteins and oligomers on membranes.
Subdiffusion of proteins and oligomers on membranes.
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DOI：
10.1063/1.4764305
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
David Lepzelter;M. Zaman
中科院分区：
文献类型：
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作者：
David Lepzelter;M. Zaman
蛋白质在脂质膜上的扩散在细胞信号传导过程中起着核心作用。从数学的角度来看，大多数膜扩散过程都可以用saffman - delbr<e:1> ck理论来解释。然而，最近的研究提出了理论框架的一个主要限制，即模型脂质膜缺乏复杂性。脂质结构域（有时被称为膜筏）被认为可以减缓蛋白质的扩散，但在一般情况下，没有定量的理论研究可以减缓多少扩散。我们提供了一个整体的理论框架，限制域（“围栏”）扩散。此外，Saffman和delbr<e:1> ck的基本结论存在多个明显的矛盾，每一个都涉及单个蛋白质或低聚物具有多个跨膜区域通过脂相屏障的情况。我们提出了一组对saffman - delbr<e:1>理论的修正来解释这些实验观察。我们的修正能够为许多细胞信号传导过程提供定量解释，这些过程被认为超出了saffman - delbr<e:1>理论的范围，并且可以扩展到其他形式的亚扩散。
Diffusion of proteins on lipid membranes plays a central role in cell signaling processes. From a mathematical perspective, most membrane diffusion processes are explained by the Saffman-Delbrück theory. However, recent studies have suggested a major limitation in the theoretical framework, the lack of complexity in the modeled lipid membrane. Lipid domains (sometimes termed membrane rafts) are known to slow protein diffusion, but there have been no quantitative theoretical examinations of how much diffusion is slowed in a general case. We provide an overall theoretical framework for confined-domain ("corralled") diffusion. Further, there have been multiple apparent contradictions of the basic conclusions of Saffman and Delbrück, each involving cases in which a single protein or an oligomer has multiple transmembrane regions passing through a lipid phase barrier. We present a set of corrections to the Saffman-Delbrück theory to account for these experimental observations. Our corrections are able to provide a quantitative explanation of numerous cellular signaling processes that have been considered beyond the scope of the Saffman-Delbrück theory, and may be extendable to other forms of subdiffusion.