CD1-restricted CD4+ T cells in major histocompatibility complex class II-deficient mice.
CD1-restricted CD4+ T cells in major histocompatibility complex class II-deficient mice.
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DOI：
10.1084/jem.182.4.993
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发表时间：
1995-10-01
期刊：
影响因子：
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通讯作者：
Mathis D
中科院分区：
文献类型：
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作者：
Cardell S;Tangri S;Chan S;Kronenberg M;Benoist C;Mathis D
更出乎意料的是，主要组织相容性复合体II类缺陷小鼠外周血中有大量的CD4+T淋巴细胞。我们在群体和克隆水平上对这些细胞进行了研究。来自II类缺陷动物的CD4+T淋巴细胞来源于胸腺，在个体发育早期出现，表现出静止记忆细胞的表型，具有多种标准的潜在功能，并具有不同的T细胞受体谱系。它们不包括显著增加的NK1.1+细胞数量。从II类缺陷动物的CD4+淋巴细胞多克隆刺激后产生的杂交瘤包括一个具有不寻常反应模式的亚群，对包括起源品系在内的许多小鼠品系的脾细胞产生反应。这个亚群中的大多数成员识别主要的组织相容性复合体Ib类分子CD1；它们的异质性反应和T细胞受体的使用进一步表明多肽和/或高度可变的翻译后修饰参与其中。
Rather unexpectedly, major histocompatibility complex class II- deficient mice have a significant population of peripheral CD4+ T lymphocytes. We have investigated these cells at the population and clonal levels. CD4+ T lymphocytes from class II-deficient animals are thymically derived, appear early in ontogeny, exhibit the phenotype of resting memory cells, are potentially functional by several criteria, and have a diverse T cell receptor repertoire. They do not include substantially elevated numbers of NK1.1+ cells. Hybridomas derived after polyclonal stimulation of the CD4+ lymphocytes from class II- deficient animals include a subset with an unusual reactivity pattern, responding to splenocytes from many mouse strains including the strain of origin. Most members of this subset recognize the major histocompatibility complex class Ib molecule CD1; their heterogeneous reactivities and T cell receptor usage further suggest the involvement of peptides and/or highly variable posttranslational modifications.