Effects of ozone treatment on gene profiling involved in ASA-GSH cycle in postharvest cantaloupe
Effects of ozone treatment on gene profiling involved in ASA-GSH cycle in postharvest cantaloupe
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臭氧处理对采后哈密瓜 ASA-GSH 循环基因谱的影响
DOI：
10.1016/j.scienta.2023.111843
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发表时间：
2023-03
影响因子：
4.3
通讯作者：
Yumei Jiang
中科院分区：
文献类型：
--
作者：
Xiaohui Lu;Huijie Zhang;Na Zhang;Chenghu Dong;Haipeng Ji;Jinze Yu;Zhaojun Ban;Ruixiang Yan;Ting Zhang;Cunkun Chen;Yumei Jiang
臭氧已被证明是一种环境友好的哈密瓜采后储存方法，可以消除活性氧（ROS），但其机制尚不清楚。本研究以关键酶活性、转录组学以及抗坏血酸（ASA）、还原性谷胱甘肽（GSH）、H2O2和（MDA）含量为典型指标，探讨臭氧处理对哈密瓜收获后抗坏血酸-谷胱甘肽（ASA-GSH）循环代谢的影响。结果表明，臭氧处理组（OT）哈密瓜H2O2和MDA的生成减少，ASA和GSH的积累增加。臭氧促进了贮藏期间哈密瓜ASA-GSH循环关键酶的活性，特别是抗坏血酸过氧化物酶（APX）、单脱氢抗坏血酸还原酶（MDHAR）和谷胱甘肽还原酶（GR）的活性，这与H2O2、MDA、ASA和GSH的变化有关。利用转录组学方法鉴定了14个与ASA-GSH循环相关的基因，结果表明，第10天，OT处理的GR-2、mmdhar -3和mmdhar -4的FPKM均高于对照组（CK），第30天，AO基因均受到刺激，说明臭氧处理可能是采后哈密瓜ASA-GSH循环的AO和MDHAR基因诱导的靶基因。相关分析表明，臭氧处理哈密瓜调节活性氧的能力是与ASA-GSH循环相关的酶活性和基因表达的协同作用。本研究为进一步研究臭氧对多种果蔬中ASA-GSH循环可能产生的影响奠定了基础。
Ozone has been proven to be an environmental-friendly method in postharvest storage of cantaloupe with the elimination of reactive oxygen species (ROS), but the mechanism is not clear. In this study, the activity of key enzymes, transcriptomics, and the content of Ascorbic acid(ASA), Reduced glutathione (GSH), H2O2, and (MDA) were measured as typical indicators to investigate the effect of ozone treatment on the ascorbic acid-glutathione (ASA-GSH) cycle metabolism of the antioxidant defense system of cantaloupe after harvest. The results showed that the formation of H2O2 and MDA and the accumulation of ASA and GSH in cantaloupe were reduced and increased in the ozone treatment group (OT), respectively. Ozone promoted the activity of the key enzymes of the ASA-GSH cycle in cantaloupe during storage, particularly Ascorbate peroxidase (APX),monodehydroascorbate reductase (MDHAR), and glutathione reductase (GR), which was confrmed with the variation of H2O2, MDA, ASA, and GSH. The 14 genes associated with the ASA-GSH cycle were identifed using transcriptomics, and the FPKM in GR-2, MDHAR-3, and MDHAR-4 were greater in OT than in the control group (CK) on the 10th day, all AO genes were stimulated in OT compared to CK on the 30th day, indicating that AO and MDHAR genes of ASA-GSH cycle may be the target genes induced by ozone treatment in the postharvest cantaloupe. Correlation analysis indicated that the ability of ozone-treated cantaloupe to regulate ROS was a synergistic effect of enzyme activity and gene expression related to the ASA-GSH cycle. This study was to lay a foundation for future studies on the possible effects of ozone on the ASA-GSH cycle of many fruit and vegetable.
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DOI：
10.4028/www.scientific.net/amr.204-210.672
发表时间：
2011-02
期刊：
Advanced Materials Research
影响因子：
--
作者：
T. Zhao;Jun Wang;Yan Wang;Ying Cao
通讯作者：
T. Zhao;Jun Wang;Yan Wang;Ying Cao
影响因子：
9.8
作者：
Fernandes, Francine Faia;Esposito, Marisia Pannia;Paoletti, Elena
通讯作者：
Paoletti, Elena
影响因子：
5.3
作者：
Mellidou I;Keulemans J;Kanellis AK;Davey MW
通讯作者：
Davey MW
影响因子：
6
作者：
Yangjin Jung;Jingwen Gao;Hyein Jang;Mengqi Guo;K. Matthews
通讯作者：
Yangjin Jung;Jingwen Gao;Hyein Jang;Mengqi Guo;K. Matthews
影响因子：
7.2
作者：
Samuel, MA;Miles, GP;Ellis, BE
通讯作者：
Ellis, BE