Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater
Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater
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DOI：
10.1128/aac.00564-17
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发表时间：
2017-09-01
影响因子：
4.9
通讯作者：
Yoneda, Minoru
中科院分区：
文献类型：
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作者：
Gomi, Ryota;Matsuda, Tomonari;Yoneda, Minoru
产超广谱β-内酰胺酶（ESBL）的大肠杆菌（ESBREC）对环境沃茨的污染引起了人们的极大关注。污水处理厂（WWTPs）和医院向环境中释放大量ESBLEC。在本研究中，我们从日本污水处理厂和医院收集的废水中分离ESBLEC菌株，并进行全基因组测序以表征这些菌株。对54株（32株来自污水处理厂，22株来自医院废水）的基因组分析显示，肠外致病性E.污水处理厂和医院废水中大肠杆菌的状况。进行精细系统发育分析，以进一步表征15个序列类型131（ST 131）复杂菌株（11个来自污水处理厂，4个来自医院废水）。这些ST 131复合菌株由以下不同的亚组组成：来自WWTP的菌株的进化枝A（n = 2）、C1-M27（n = 8）和C1（非C1-M27）（n = 1），来自医院废水的菌株的进化枝A（n = 2）、C1-M27（n = 1）和C1（非C1-M27）（n = 1）。结果表明，ESBLEC菌株属于临床上重要的谱系，包括C1-M27分支，可能通过废水传播到环境中，这突出了监测废水中抗生素耐药性的必要性。
Contamination of environmental waters by extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli (ESBLEC) is of great concern. Wastewater treatment plants (WWTPs) and hospitals release large amounts of ESBLEC into the environment. In the present study, we isolated ESBLEC strains from wastewater collected from a WWTP and a hospital in Japan and performed whole-genome sequencing to characterize these strains. Genomic analysis of 54 strains (32 from the WWTP and 22 from hospital wastewater) revealed the occurrence of clinically important clonal groups with extraintestinal pathogenic E. coli status in the WWTP and hospital wastewater. Fine-scale phylogenetic analysis was performed to further characterize 15 sequence type 131 (ST131) complex strains (11 from the WWTP and 4 from hospital wastewater). These ST131 complex strains were comprised of the following different subgroups: clade A (n = 2), C1-M27 (n = 8), and C1 (non-C1-M27) (n = 1) for strains from the WWTP and clade A (n = 2), C1-M27 (n = 1), and C1 (non-C1-M27) (n = 1) for strains from hospital wastewater. The results indicate that ESBLEC strains belonging to clinically important lineages, including the C1-M27 clade, may disseminate into the environment through wastewater, highlighting the need to monitor for antibiotic resistance in wastewater.