Identification and disruption of the gene encoding the K(+)-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae.
Identification and disruption of the gene encoding the K(+)-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae.
复制标题
编码酿酒酵母 K( )激活乙醛脱氢酶的基因的鉴定和破坏。
DOI：
10.1111/j.1574-6968.1998.tb13063.x
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发表时间：
1998
影响因子：
2.1
通讯作者：
M. Midgley
中科院分区：
文献类型：
--
作者：
W. Tessier;P. Meaden;F. Dickinson;M. Midgley
编码酿酒酵母线粒体 K(+) 激活乙醛脱氢酶 (K(+)-ACDH) 的基因的身份已得到证实。该基因位于XV染色体的右臂，系统名称为YOR374w，推导的产物与酿酒酵母醛脱氢酶(ALDH)家族的其他成员显示出显着的同源性。 YOR374w 现已被指定为基因名称 ALD7。测定了从几种不同的酿酒酵母菌株纯化的K(+)-ACDH的N端氨基酸序列，发现与ALD7的产物比对具有81-100%的同一性。构建了含有 ALD7 缺失的单倍体突变体，并且在这些菌株中，在所检查的任何生长条件下都检测不到 K(+)-ACDH。由ALD6编码的Mg(2+)激活的乙醛脱氢酶(Mg(2+)-ACDH)的活性在突变体中保持在野生型水平。缺乏 ALD7 的突变体在葡萄糖上的生长未受影响（与 ald6 突变体的行为相反），而在乙醇上的生长受到严重损害。这一观察结果与我们小组之前的工作一起表明，Mg(2+)-和K(+)-ACDH都是在乙醇上生长所必需的，而只有前者在葡萄糖上生长期间发挥作用。
The identity of the gene encoding the mitochondrial K(+)-activated acetaldehyde dehydrogenase (K(+)-ACDH) of Saccharomyces cerevisiae has been confirmed. The gene is situated on the right arm of chromosome XV, bears the systematic name YOR374w and the deduced product shows significant homology to other members of the S. cerevisiae aldehyde dehydrogenase (ALDH) family. YOR374w has now been assigned the gene name ALD7. The N-terminal amino acid sequences of K(+)-ACDHs purified from several diverse strains of S. cerevisiae were determined, and found to have 81-100% identity in alignments with the product of ALD7. Haploid mutants containing a deletion of ALD7 were constructed and, in these strains, the K(+)-ACDH was not detectable under any growth conditions examined. The activity of the Mg(2+)-activated acetaldehyde dehydrogenase (Mg(2+)-ACDH), encoded by ALD6, remained at wild-type levels in the mutants. Growth on glucose was not affected in the mutants lacking ALD7 (in contrast to the behaviour of ald6 mutants), whereas growth on ethanol was severely impaired. This observation, together with previous work by our group, shows that both the Mg(2+)- and K(+)-ACDHs are required for growth on ethanol, whilst only the former plays a role during growth on glucose.