A biomimetic olfactory-based biosensor with high efficiency immobilization of molecular detectors.
A biomimetic olfactory-based biosensor with high efficiency immobilization of molecular detectors.
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DOI：
10.1016/j.bios.2011.09.037
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发表时间：
2012-01
影响因子：
12.6
通讯作者：
Chunsheng Wu;L. Du;Di Wang;Luhang Zhao;Ping Wang
中科院分区：
文献类型：
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作者：
Chunsheng Wu;L. Du;Di Wang;Luhang Zhao;Ping Wang
分子检测器的固定化效率对生物传感器的灵敏度、稳定性和重现性等性能具有重要影响。本文通过提高分子检测器的固定化效率，提出了一种性能更好的仿生嗅觉受体生物传感器。在声表面波（SAW）芯片的敏感区域上构建了一种具有特定嗅觉受体（ODR-10）功能的混合自组装单分子膜（SAMs）。利用扫描电镜（SEM）和原子力显微镜（AFM）对ODR-10的固定化进行了表征。该传感器对各种气味物的响应通过监测SAW的共振频率偏移来记录，SAW的共振频率偏移与其敏感区域上的质量负载相关。结果表明，该传感器对ODR-10的天然配体双乙酰具有特异性响应，具有较高的灵敏度和稳定性。灵敏度为4kHz/ng，比以前的工作提高了2倍。检测限为1.2×10− 11 mM。本工作在分子检测器固定化效率方面取得的重大进展将极大地促进和加速基于嗅觉受体的生物传感器的研究和应用，这些传感器包括石英晶体微天平（QCM）、表面等离子体共振（SPR）和场效应晶体管（FET）。
The immobilization efficiency of molecular detectors is of great importance with regard to the performances of biosensors such as the sensitivity, stability, and reproducibility. This paper presents a biomimetic olfactory receptor-based biosensor with better performances by improving the immobilization efficiency of molecular detectors for odorant sensing. A mixed self-assembled monolayers (SAMs) functionalized with specific olfactory receptors (ODR-10) was constructed on the sensitive area of surface acoustic wave (SAW) chip. The immobilization of ODR-10 was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The responses of this biosensor to various odorants were recorded by monitoring the resonance frequency shifts of SAW, which is correlated to the mass loading on its sensitive area. All the results demonstrate this biosensor can specifically respond to the natural ligand of ODR-10, diacetyl, with high sensitivity and stability. The sensitivity is 4kHz/ng, which is 2× higher than that of previous work. The detection limit is 1.2×10−11mM. The major advances on immobilization efficiency of molecular detectors presented in this work could substantially promote and accelerate the researches and applications of olfactory receptor-based biosensors with different transducers, such as quartz crystal microbalance (QCM), surface plasma resonance (SPR), and field effect transistors (FET).