Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica.
Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica.
复制标题
DOI：
10.4238/2015.october.2.16
复制
发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
H. Y. Yang;X. Xia;W. Fang;Y. Fu;M. An;M. Zhou
中科院分区：
文献类型：
--
作者：
H. Y. Yang;X. Xia;W. Fang;Y. Fu;M. An;M. Zhou
竹子叶色的自发变异为研究叶色形成机制和观赏竹育种提供了契机。尽管在模式植物中已知许多基因与叶色变异有关，但竹子自然叶色变异的分子机制仍不清楚。利用抑制性消减杂交（SSH）技术，对日本竹（Pseudosasajaponica f.）的绿色叶和白化病叶进行分析，以筛选出导致这一现象的基因。从正向和反向SSH文库中分别获得1062和1004个差异表达转录本。随后，选择了59个差异表达的unigene，其在叶色形成中具有潜在作用，通过计算分析其功能相关性进行预测，用于使用qPCR进行进一步分析。10个基因，参与光合作用，质体发育，阳离子信号转导，表现出2倍的表达水平之间的变化绿色和白化病叶。进一步分析这些基因在三个发育阶段的表达模式发现，Lhca 1编码的叶绿素a/B结合蛋白在白化病叶片中的表达丰度比在绿色叶片中低得多。我们的研究结果表明，连同随后的光合作用过程的串联负压，白化病表型至少部分归因于叶绿体内膜损伤或光合色素积累的损害，这是从低Lhca 1表达的结果。
Spontaneous leaf color variation in bamboo provides the opportunity to study the mechanisms of leaf color formation and the breeding of ornamental bamboos. Despite the fact that many genes are known to be involved in leaf color variation in model plants, molecular mechanisms governing natural leaf color variation in bamboo have remained obscure. This study aimed to identify the genes responsible for the occurrence of such phenomena in bamboo using the suppression subtractive hybridization (SSH) method between green and albino leaves in Pseudosasa japonica f. A total of 1062 and 1004 differentially expressed transcripts were obtained from the forward and reverse SSH libraries, respectively. Subsequently, 59 differentially expressed unigenes with potential roles in leaf color formation, predicted via computational analysis of their functional relevance, were selected for further analysis using qPCR. Ten genes, involved in photosynthesis, plastid development, and cation signal transduction, showed 2-fold changes in expression levels between green and albino leaves. Further expression pattern analyses of these genes at three developmental stages revealed much lower expression abundance of Lhca1-encoded chlorophyll a/b binding protein in the albino leaves than in the green leaves. Our results suggest that, together with the concatenated negative pressure for subsequent photosynthetic processes, the albino phenotype is at least partly attributable to chloroplast inner membrane damage or to the impairment of photosynthetic pigment accumulation, which results from low Lhca1 expression.