Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma
Proteomic Analysis of Differentially Expressed Proteins in Peripheral Cholangiocarcinoma
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DOI：
10.1007/s12307-010-0047-2
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发表时间：
2011-04-01
影响因子：
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通讯作者：
Desmouliere, Alexis
中科院分区：
文献类型：
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作者：
Darby, Ian A.;Vuillier-Devillers, Karine;Desmouliere, Alexis
胆管癌是一种肝腺癌，在过去三十年中发病率增加，达到与其他肝癌相似的水平。该病的诊断通常较晚且预后差，因此鉴定新的候选标志物和潜在的早期指标以及可能成为潜在治疗靶点的分子是非常重要的。我们使用蛋白质组学方法来鉴定周围胆管癌病例中差异表达的蛋白质，并与来自相同患者的配对非肿瘤肝组织的表达进行比较。用花青3和花青5标记蛋白质后进行二维荧光差异凝胶电泳，以鉴定差异表达的蛋白质。总的来说，在每个凝胶中观察到的大约2,400个蛋白质点中，172个蛋白质点在肿瘤和非肿瘤组织之间的表达水平显示出显著差异，
Cholangiocarcinoma is an adenocarcinoma of the liver which has increased in incidence over the last thirty years to reach similar levels to other liver cancers. Diagnosis of this disease is usually late and prognosis is poor, therefore it is of great importance to identify novel candidate markers and potential early indicators of this disease as well as molecules that may be potential therapeutic targets. We have used a proteomic approach to identify differentially expressed proteins in peripheral cholangiocarcinoma cases and compared expression with paired non-tumoral liver tissue from the same patients. Two-dimensional fluorescence difference gel electrophoresis after labeling of the proteins with cyanines 3 and 5 was used to identify differentially expressed proteins. Overall, of the approximately 2,400 protein spots visualised in each gel, 172 protein spots showed significant differences in expression level between tumoral and non-tumoral tissue with p