CEREBELLAR NUCLEI AND THE NUCLEOCORTICAL PROJECTIONS IN THE RAT - RETROGRADE TRACING COUPLED TO GABA AND GLUTAMATE IMMUNOHISTOCHEMISTRY
CEREBELLAR NUCLEI AND THE NUCLEOCORTICAL PROJECTIONS IN THE RAT - RETROGRADE TRACING COUPLED TO GABA AND GLUTAMATE IMMUNOHISTOCHEMISTRY
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DOI：
10.1002/cne.903150106
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发表时间：
1992-01-01
影响因子：
2.5
通讯作者：
GUEGAN, M
中科院分区：
文献类型：
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作者：
BATINI, C;COMPOINT, C;GUEGAN, M
氨基酸GABA和谷氨酸（Glu）被认为是中枢神经系统中负责神经元抑制和兴奋的主要物质。 因此，它们在定义的通路中的不同神经元之间的分布可以指示细胞对通路功能的贡献。 这种神经元的例子是小脑核，同时调节小脑皮质的浦肯野细胞的输出，也被发现项目回小脑cortic. Immunohistochemistry实验，以确定GABA和谷氨酸（Glu）的细胞在成年大鼠小脑核。 将连续的半薄切片和连续的振动切片与兔抗GABA和Glu的抗血清孵育。 在半薄切片中，仅小神经元呈GABA阳性（31.7%），大部分（80.5%）呈不同大小的Glu阳性（80.5%）。 与Glu是GABA的代谢前体一致，75.4%的GABA-IR群体共定位于Glu。 在vibratome节GABA-IR神经元的数量表现出一些局部差异，而Glu-IR均匀分布在三个核研究。 测量的这些神经元的平均直径显示GABA-和Glu-IR的明显尺寸差异，几乎没有重叠。通过局部预注射逆行转运的WGA-apoHRP-胶体金复合物来鉴定投射到皮质的小脑核神经元（核皮质神经元，NCN）在小脑皮质中。 这些小脑的振动切片用银强化逆行示踪剂，并用GABA和Glu双重标记。 在识别的NCN总数中，8.7%为GABA-IR（10只动物），47.7%为Glu-IR（5只动物）。 由于抗体渗透性差，厚切片核心中的许多逆行标记的NCN对两种氨基酸均呈免疫阴性，因此低估了含有GABA和Glu的细胞的比例。 GABA-IR和Glu-IR NCN的大小分布与厚切片中非逆行标记核的大小分布相似，得出的结论是，小脑核（包括NCN）的GABA-IR神经元使用GABA作为假定的抑制性神经递质，而Glu-IR神经元可能使用Glu或其他兴奋性神经递质。
The amino acids GABA and glutamate (Glu) are thought to be the principal substances in the central nervous system responsible for neuronal inhibition and excitation. Their distributions among the different neurons in a defined pathway may thus be indicative of the contributions of the cells to pathway function. Examples of such neurons are those of the cerebellar nuclei which, while regulating output from the Purkinje cells of the cerebellar cortex, are also found to project back to the cerebellar cortex.Immunohistochemical experiments were done to identify GABA and glutamate (Glu) containing cells in the adult rat cerebellar nuclei. Consecutive semithin and serial vibratome sections were incubated with antisera raised in rabbit against GABA and Glu. In semithin sections, only small neurons were intensely GABA immunoreactive (GABA-IR) (31.7%), and the majority (80.5%) were Glu immunoreactive (Glu-IR) of different sizes. Consistent with Glu being a metabolic precursor for GABA, 75.4% of the GABA-IR population colocalized Glu. In vibratome sections GABA-IR neurons showed some local differences in number, whereas the Glu-IR were uniformly distributed in the three nuclei studied. Measured mean diameters for these neurons showed a distinct size difference for the GABA- and Glu-IR with little overlap.Cerebellar nuclei neurons projecting to the cortex (nucleocortical neurons, NCN) were identified by locally preinjecting the retrograde transported WGA-apoHRP-colloidal gold complex in the cerebellar cortex. Vibratome sections of these cerebella were silver intensified for the retrograde tracer and double labeled for GABA and Glu. Of the total number of identified NCN, 8.7% were GABA-IR (10 animals) and 47.7% Glu-IR (5 animals). Many retrograde labeled NCN in the core of the thick sections were immunonegative for both amino acids due to poor antibody penetration, thus underestimating the proportions of cells containing GABA and Glu. The size distributions for the GABA-IR and Glu-IR NCN were similar to those measured in non-retrograde labeled nuclei in thick sections.The conclusions reached are that GABA-IR neurons of the cerebellar nuclei, including the NCN, use GABA as the presumed inhibitory neurotransmitter and that Glu-IR neurons may use Glu or another excitatory neurotransmitter.