RNA sequencing revealed the abnormal transcriptional profile in cloned bovine embryos.
RNA sequencing revealed the abnormal transcriptional profile in cloned bovine embryos.
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RNA测序揭示了克隆牛胚胎中的异常转录谱
DOI：
10.1016/j.ijbiomac.2020.02.026
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Zekun Guo
中科院分区：
文献类型：
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作者：
Lei Zhang;Mengying Yu;Hongyu Xu;Xing Wei;Yingxiang Liu;Chenyang Huang;Huanhuan Chen;Zekun Guo
体细胞核移植技术在农业和生物医学领域具有潜在的应用前景，但其克隆效率仍然较低。在这项研究中，克隆胚胎和受精胚胎的转录谱测量和比较RNA测序。检测2细胞胚胎，以确定IVF和SCNT获得的胚胎之间最早的转录差异。结果显示，364个基因在克隆的2细胞胚胎中表达减少，并在“细胞内蛋白转运”和“泛素介导的蛋白水解”中富集。在囊胚中，593个基因在克隆囊胚中表达减少，在“RNA结合”、“核苷酸结合”、“胚胎发育”和“粘附体连接”中富集。我们鉴定出14个在克隆胚胎中未被激活的发育相关基因。然后，在克隆的2细胞胚胎和克隆的囊胚中分别识别出68和245个长链非编码rna的异常表达。此外，我们发现克隆胚胎中发生了不完全的rna编辑，这可能是由ADAR表达降低引起的。总之，我们的研究揭示了克隆胚胎中异常转录本和缺乏rna编辑位点，为进一步研究体细胞核重编程的机制提供了新的数据。
Somatic cell nuclear transfer (SCNT) has potential applications in agriculture and biomedicine, but the efficiency of cloning is still low. In this study, the transcriptional profiles in cloned and fertilized embryos were measured and compared by RNA sequencing. The 2-cell embryos were detected to identify the earliest transcriptional differences between embryos derived through IVF and SCNT. As a result, 364 genes showed decreased expression in cloned 2-cell embryos and were enriched in "intracellular protein transport" and "ubiquitin mediated proteolysis". In blastocysts, 593 genes showed decreased expression in cloned blastocysts and were enriched in "RNA binding", "nucleotide binding", "embryo development", and "adherens junction". We identified 14 development related genes that were not activated in the cloned embryos. Then, 68 and 245 long non-coding RNAs were recognized abnormally expressed in cloned 2-cell embryos and cloned blastocysts, respectively. Furthermore, we found that incomplete RNA-editing occurred in cloned embryos and might be caused by decreased ADAR expression. In conclusion, our study revealed the abnormal transcripts and deficient RNA-editing sites in cloned embryos and provided new data for further mechanistic studies of somatic nuclear reprogramming.