A molecular phylogeographic study based on DNA sequences from individual metacercariae of Paragonimus mexicanus from Guatemala and Ecuador
A molecular phylogeographic study based on DNA sequences from individual metacercariae of Paragonimus mexicanus from Guatemala and Ecuador
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DOI：
10.1079/joh2002147
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发表时间：
2003-03-01
影响因子：
1.6
通讯作者：
Agatsuma, T
中科院分区：
文献类型：
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作者：
Iwagami, M;Monroy, C;Agatsuma, T
对采自危地马拉和厄瓜多尔的墨西哥并殖吸虫进行了分子生物地理学研究。从单个囊蚴中提取基因组DNA，并通过聚合酶链反应（PCR）扩增两个基因区域（部分线粒体细胞色素c氧化酶亚基1（CO1）和核核糖体基因重复序列的第二内转录间隔区（ITS2））。根据其地理起源在系统发生树中分离的序列。来自厄瓜多尔和危地马拉的ITS2序列仅在一个位点上不同。一个国家内的CO1序列之间的成对距离总是低于国家之间。然而，国家之间的遗传距离小于已被认为是不同物种的卫氏肺吸虫地理类型之间的遗传距离。这一结果表明，危地马拉和厄瓜多尔的种群可能在亚种水平上存在遗传差异。
A molecular phylogeographic study of Paragonimus mexicanus collected from Guatemala and Ecuador was performed. Genomic DNA was extracted from individual metacercariae, and two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (CO1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (ITS2)) were amplified by the polymerase chain reaction (PCR). Sequences segregated in a phylogenetic tree according to their geographic origins. ITS2 sequences from Ecuador and Guatemala differed at only one site. Pairwise distances among CO1 sequences within a country were always lower than between countries. Nevertheless, genetic distances between countries were less than between geographical forms of P. westermani that have been suggested to be distinct species. This result suggests that populations from Guatemala and Ecuador are genetically differentiated perhaps at the level of subspecies.