Identification of key genes in benign prostatic hyperplasia using bioinformatics analysis
Identification of key genes in benign prostatic hyperplasia using bioinformatics analysis
复制标题
利用生物信息学分析鉴定良性前列腺增生的关键基因
DOI：
10.1007/s00345-021-03625-5
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发表时间：
2021-02-09
影响因子：
3.4
通讯作者：
Ping, Hao
中科院分区：
文献类型：
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作者：
Xiang, Peng;Liu, Dan;Ping, Hao
目的通过综合生物信息学分析，探讨良性前列腺增生（BPH）的差异表达基因（DEGs）及其表达途径。方法从GEO数据库下载基因表达芯片（GSE6099）的数据。用GEO2R测定deg。选择的基因在DAVID数据库中进行功能和富集分析。通过STRING构建蛋白-蛋白相互作用网络。采用免疫组化（IHC）方法检测前列腺增生和正常前列腺石蜡包埋标本的前路梯度2 （ARG2）和lumican （LUM）染色。通过学生测试分析各组之间的差异。结果共检测到24个上皮蛋白和39个间质蛋白。GO分析结果显示，BPH和NP之间的上皮deg在葡萄糖代谢过程、葡萄糖稳态和Rho蛋白信号转导的负调控等生物学过程中富集。对于基质中的DEGs，富集的生物过程包括对缺血的反应、抗原加工和递呈、软骨发育、T细胞共刺激和能量储备代谢过程。ARG2作为上皮deg之一，主要位于前列腺上皮细胞中。此外，LUM主要在基质中表达。通过免疫组化进一步证实，与NP相比，BPH具有较低的ARG2蛋白水平（p= 0.029）和较高的LUM蛋白水平（p= 0.003）。结论sour研究提示上皮细胞和基质细胞中可能存在ARG2、LUM等差异表达基因，为BPH的发病机制提供了新的思路。
PurposeThis study aimed to identify differentially expressed genes (DEGs) and pathways in benign prostatic hyperplasia (BPH) by comprehensive bioinformatics analysis.MethodsData of the gene expression microarray (GSE6099) were downloaded from GEO database. DEGs were obtained by GEO2R. Functional and enrichment analyses of selected genes were performed using DAVID database. Protein–protein interaction network was constructed through STRING. Anterior gradient 2 (ARG2) and lumican (LUM) staining in paraffin-embedded specimens from BPH and normal prostate (NP) were detected by immunohistochemistry (IHC). Differences between groups were analyzed by the Student’sttest.ResultsA total of 24 epithelial DEGs and 39 stromal DEGs were determined. The GO analysis results showed that epithelial DEGs between BPH and NP were enriched in biological processes of glucose metabolic process, glucose homeostasis and negative regulation of Rho protein signal transduction. For DEGs in stroma, enriched biological processes included response to ischemia, antigen processing and presentation, cartilage development, T cell costimulation and energy reserve metabolic process. ARG2, as one of the epithelial DEGs, was mainly located in epithelial cells of prostate. In addition, LUM is primarily expressed in the stroma. We further confirmed that compared with NP, the BPH have the lower ARG2 protein level (p= 0.029) and higher LUM protein level (p= 0.003) using IHC.ConclusionsOur study indicated that there are possible differentially expressed genes in epithelial and stromal cells, such as ARG2 and LUM, which may provide a novel insight for the pathogenesis of BPH.