Dethiosulfatibacter aminovorans gen. nov., sp. nov., a novel thiosulfate-reducing bacterium isolated from coastal marine sediment via sulfate-reducing enrichment with Casamino acids.
Dethiosulfatibacter aminovorans gen. nov., sp. nov., a novel thiosulfate-reducing bacterium isolated from coastal marine sediment via sulfate-reducing enrichment with Casamino acids.
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DOI：
10.1099/ijs.0.64882-0
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发表时间：
2007-10
影响因子：
2.8
通讯作者：
S. Takii;S. Hanada;H. Tamaki;Yutaka Ueno;Y. Sekiguchi;A. Ibe;K. Matsuura
中科院分区：
文献类型：
--
作者：
S. Takii;S. Hanada;H. Tamaki;Yutaka Ueno;Y. Sekiguchi;A. Ibe;K. Matsuura
以酪蛋白氨基酸为底物，成功地建立了一种来源于日本东京湾富营养化海洋沉积物的硫酸盐还原富集培养物。经谷氨酸富集后，从富集培养物中分离得到一株硫代硫酸盐还原菌C/G2（T）。菌株C/G2（T）的细胞为非运动的杆状（0.6-0.8 μ m × 2.2-4.8 μ m），单个或成对存在，有时呈短链。孢子没有形成。菌株C/G2（T）的细胞尽管具有革兰氏阳性细胞壁，但仍染色为革兰氏阴性。生长的最适温度为28-30 ℃，最适pH为7.8左右，最适盐浓度为20-30 g l（-1）。乳酸盐、丙酮酸盐、丝氨酸、半胱氨酸、苏氨酸、谷氨酸盐、组氨酸、赖氨酸、精氨酸、酪蛋白氨基酸、蛋白胨和酵母提取物作为单一底物发酵，并且不使用糖作为发酵底物。观察到与某些氨基酸对的Stickland反应。富马酸、丙氨酸、脯氨酸、苯丙氨酸、色氨酸、谷氨酰胺和天冬氨酸仅在硫代硫酸盐存在下使用。菌株C/G2（T）发酵谷氨酸产生H2、CO2、乙酸和丙酸。硫代硫酸盐和元素硫被还原成硫化物。硫酸盐、亚硫酸盐和硝酸盐未被用作电子受体。通过与从富含酪蛋白氨基酸的原始混合培养物中分离的脱硫弧菌（Desulfovibrio sp.）共培养，菌株C/G2（T）在酪蛋白氨基酸或谷氨酸上的生长得到增强。菌株C/G2（T）的DNA G+C含量为41.0mol%。基于16 S rRNA基因序列的系统发育分析表明，菌株C/G2（T）与沉降杆菌属（Sedimentibacter）的几个种形成了明显的聚类。最近的亲缘关系是羟基苯甲酸沉降杆菌（基因序列相似性为91%）。根据菌株C/G2（T）（=JCM 13356（T）=NBRC 101112（T）=DSM 17477（T））的系统发育和表型特征，提出菌株C/G2（T）代表一个新属和新种，即食氨脱硫硫酸杆菌（Dethiosulfatibacter aminovorans gen. nov.）sp. Nov.
A sulfate-reducing enrichment culture originating from coastal marine sediment of the eutrophic Tokyo Bay, Japan, was successfully established with Casamino acids as a substrate. A thiosulfate reducer, strain C/G2(T), was isolated from the enrichment culture after further enrichment with glutamate. Cells of strain C/G2(T) were non-motile rods (0.6-0.8 microm x 2.2-4.8 microm) and were found singly or in pairs and sometimes in short chains. Spores were not formed. Cells of strain C/G2(T) stained Gram-negatively, despite possessing Gram-positive cell walls. The optimum temperature for growth was 28-30 degrees C, the optimum pH was around 7.8 and the optimum salt concentration was 20-30 g l(-1). Lactate, pyruvate, serine, cysteine, threonine, glutamate, histidine, lysine, arginine, Casamino acids, peptone and yeast extract were fermented as single substrates and no sugar was used as a fermentative substrate. A Stickland reaction was observed with some pairs of amino acids. Fumarate, alanine, proline, phenylalanine, tryptophan, glutamine and aspartate were utilized only in the presence of thiosulfate. Strain C/G2(T) fermented glutamate to H2, CO2, acetate and propionate. Thiosulfate and elemental sulfur were reduced to sulfide. Sulfate, sulfite and nitrate were not utilized as electron acceptors. The growth of strain C/G2(T) on Casamino acids or glutamate was enhanced by co-culturing with Desulfovibrio sp. isolated from the original mixed culture enriched with Casamino acids. The DNA G+C content of strain C/G2(T) was 41.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C/G2(T) formed a distinct cluster with species of the genus Sedimentibacter. The closest relative was Sedimentibacter hydroxybenzoicus (with a gene sequence similarity of 91 %). On the basis of its phylogenetic and phenotypic properties, strain C/G2(T) (=JCM 13356(T)=NBRC 101112(T)=DSM 17477(T)) is proposed as representing a new genus and novel species, Dethiosulfatibacter aminovorans gen. nov., sp. nov.