Development of polarization-sensitive optical coherence tomography imaging platform and metrics to quantify electrostimulation-induced peripheral nerve injury in vivo in a small animal model.
Development of polarization-sensitive optical coherence tomography imaging platform and metrics to quantify electrostimulation-induced peripheral nerve injury in vivo in a small animal model.
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DOI：
10.1117/1.nph.10.2.025004
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发表时间：
2023-04
期刊：
影响因子：
5.3
通讯作者：
中科院分区：
文献类型：
--
作者：
神经调节装置正在迅速发展，用于治疗神经系统疾病和病症。无明显功能损失的植入或长期使用造成的损伤通常只能通过终末组织学检测到。需要新的技术来评估正常和患病或受伤情况下的周围神经系统（PNS）。我们的目标是展示一个成像和刺激平台，可以阐明神经刺激在PNS中的生物学机制和影响，并将其应用于坐骨神经，以提取指示电过度刺激的成像指标。利用新开发的成像和刺激平台观察了15只大鼠的坐骨神经损伤模型，该平台可以通过偏振敏感光学相干断层扫描检测电过度刺激效应。坐骨神经用电刺激1小时，随后1小时的恢复期，以高于阈值的Shannon模型值在实验组中传递：假对照组（SC,,），刺激水平1 （SL1,，和），刺激水平2 （SL2,，和）。刺激和成像系统成功地捕获了整个队列的研究数据。与恢复1周后的SC相比，最靠近刺激导联的神经束在相位延迟和光学衰减方面的平均变化（SL1/SL2）相对于SC。免疫组化（IHC）分析显示髓鞘像素计数和轴突像素计数的差异，以及细胞核像素计数的总体增加。这些指标与免疫组化和苏木精/伊红组织切片分析一致。在我们的研究中观察到的刺激后的变化是神经损伤和修复的表现，特别是变性和血管生成。光学成像指标量化这些过程，并可能有助于评估神经调节装置的安全性和有效性。
Neuromodulation devices are rapidly evolving for the treatment of neurological diseases and conditions. Injury from implantation or long-term use without obvious functional losses is often only detectable through terminal histology. New technologies are needed that assess the peripheral nervous system (PNS) under normal and diseased or injured conditions. We aim to demonstrate an imaging and stimulation platform that can elucidate the biological mechanisms and impacts of neurostimulation in the PNS and apply it to the sciatic nerve to extract imaging metrics indicating electrical overstimulation. A sciatic nerve injury model in a 15-rat cohort was observed using a newly developed imaging and stimulation platform that can detect electrical overstimulation effects with polarization-sensitive optical coherence tomography. The sciatic nerve was electrically stimulated using a custom-developed nerve holder with embedded electrodes for 1 h, followed by a 1-h recovery period, delivered at above-threshold Shannon model -values in experimental groups: sham control (SC, , ), stimulation level 1 (SL1, , , and ), and stimulation level 2 (SL2, , , and ). The stimulation and imaging system successfully captured study data across the cohort. When compared to a SC after a 1-week recovery, the fascicle closest to the stimulation lead showed an average change of (SL1/SL2) in phase retardation and in optical attenuation relative to SC. Analysis of immunohistochemistry (IHC) shows a difference in myelin pixel counts and difference in axon pixel counts, and an overall increase in cell nuclei pixel count of . These metrics were consistent with IHC and hematoxylin/eosin tissue section analysis. The poststimulation changes observed in our study are manifestations of nerve injury and repair, specifically degeneration and angiogenesis. Optical imaging metrics quantify these processes and may help evaluate the safety and efficacy of neuromodulation devices.
DOI：
10.1186/1749-7221-7-7
发表时间：
2012-10-10
影响因子：
0.7
作者：
Maripuu A;Björkman A;Björkman-Burtscher IM;Mannfolk P;Andersson G;Dahlin LB
通讯作者：
Dahlin LB
影响因子：
3.9
作者：
Tereshenko V;Pashkunova-Martic I;Manzano-Szalai K;Friske J;Bergmeister KD;Festin C;Aman M;Hruby LA;Klepetko J;Theiner S;Klose MHM;Keppler B;Helbich TH;Aszmann OC
通讯作者：
Aszmann OC