Genome-wide microRNA profiling of bovine milk-derived exosomes infected with Staphylococcus aureus
Genome-wide microRNA profiling of bovine milk-derived exosomes infected with Staphylococcus aureus
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DOI：
10.1007/s12192-018-0876-3
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发表时间：
2018-07-01
影响因子：
3.8
通讯作者：
Lai, Songjia
中科院分区：
文献类型：
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作者：
Cai, Mingcheng;He, Hongbing;Lai, Songjia
牛乳中含有丰富的外泌体，外泌体中含有丰富的miRNAs，在新生儿生长发育和获得性免疫发育中发挥重要作用。本文分析了3头健康奶牛和3头乳腺炎奶牛乳汁外泌体的miRNA表达谱，构建了6个miRNA文库。有趣的是，我们在牛奶外泌体中没有检测到scRNA和很少的snRNA;这一结果表明牛奶外泌体中RNA包装的潜在偏好。共检测到492种已知的和980种新的外泌体miRNA，6个样品中表达最多的10种miRNA占总miRNA相关读段的80-90%。表达分析鉴定了18个在健康和感染动物之间具有显著差异表达的miRNAs;差异表达的miRNAs的预测靶基因在免疫系统过程、对刺激的响应、生长等中显著富集。此外，靶基因在包括炎症、免疫和癌症途径的几个京都基因和基因组百科全书（KEGG）途径中显著富集。我们的调查提供了有关乳腺炎中涉及的牛奶外泌体和外泌体miRNA的全面信息。此外，差异表达的miRNAs，特别是miR-223和miR-142- 5 p，可以被认为是乳腺炎的潜在候选者。
Bovine milk is rich in exosomes, which contain abundant miRNAs and play important roles in the regulation of neonatal growth and development of adaptive immunity. Here, we analyzed miRNA expression profiles of bovine milk exosomes from three healthy and three mastitic cows, and then six miRNA libraries were constructed. Interestingly, we detected no scRNAs and few snRNAs in milk exosomes; this result indicated a potential preference for RNA packaging in milk exosomes. A total of 492 known and 980 novel exosomal miRNAs were detected, and the 10 most expressed miRNAs in the six samples accounted for 80-90% of total miRNA-associated reads. Expression analyses identified 18 miRNAs with significantly different expression between healthy and infected animals; the predicted target genes of differentially expressed miRNAs were significantly enriched in immune system process, response to stimulus, growth, etc. Moreover, target genes were significantly enriched in several Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including inflammatory, immune, and cancer pathways. Our survey provided comprehensive information about milk exosomes and exosomal miRNAs involved in mastitis. Moreover, the differentially expressed miRNAs, especially miR-223 and miR-142-5p, could be considered as potential candidates for mastitis.