Cholesterol induced asymmetry in DOPC bilayers probed by AFM force spectroscopy
Cholesterol induced asymmetry in DOPC bilayers probed by AFM force spectroscopy
复制标题
DOI：
10.1016/j.bbamem.2018.01.021
复制
发表时间：
2018-05-01
影响因子：
3.4
通讯作者：
Murthy, Appala Venkata Ramana
中科院分区：
文献类型：
--
作者：
Adhyapak, Pranav R.;Panchal, Suresh V.;Murthy, Appala Venkata Ramana
胆固醇对人工脂双层的机械效应是众所周知的，并已被AFM力谱彻底研究。然而，在不同的胆固醇浓度下，胆固醇进入双层的动力学及其影响还不是很清楚。在这篇文章中，我们提出了胆固醇作为其浓度的函数在一个简单的单组分二油酰磷脂酰胆碱(DOPC)双层膜中的影响。随着胆固醇的加入，在双层上测量到的突破力的性质表明，这不仅是增加机械稳定性的责任，而且还会在双层的薄片上引入不规则性。这种胆固醇引起的跨双层(内外叶)的不对称性与小叶间的耦合现象有关，并且是胆固醇浓度的函数。AFM探测的胆固醇浓度可以为脂膜的机械性质提供前所未有的方向，因为它可以直接与细胞膜的生物物理性质相关。
Cholesterol induced mechanical effects on artificial lipid bilayers are well known and have been thoroughly investigated by AFM force spectroscopy. However, dynamics of cholesterol impingement into bilayers at various cholesterol concentrations and their effects have not been clearly understood. In this paper we present, the effect of cholesterol as a function of its concentration in a simple single component dioleoylphosphatidylcholine (DOPC) bilayer. The nature of measured breakthrough forces on a bilayer with the addition of cholesterol, suggested that it is not just responsible to increase the mechanical stability but also introduces irregularities across the leaflets of the bilayer. This cholesterol induced asymmetry across the (in the inner and outer leaflets) bilayer is related to the phenomena of interleaflet coupling and is a function of cholesterol concentration probed by AFM can provide an unprecedented direction on mechanical properties of lipid membrane as it can be directly correlated to biophysical properties of a cell membrane.