Successful intrauterine insemination of Eld's deer (Cervus eldi thamin) with frozen-thawed spermatozoa.
Successful intrauterine insemination of Eld's deer (Cervus eldi thamin) with frozen-thawed spermatozoa.
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使用冻融精子成功对坡鹿 (Cervus eldi thamin) 进行宫内授精。
DOI：
10.1530/jrf.0.0990459
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
W. Rall
中科院分区：
文献类型：
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作者：
S. Monfort;G. Asher;D. Wildt;T. C. Wood;M. Schiewe;L. Williamson;Mitchell Bush;W. Rall
本研究测试了辅助生殖（同步发情和宫内人工授精（AI））对濒危物种坡鹿（Cervus eldi thamin）圈养繁殖的影响。精液是根据未充分代表的基因型预先选择的雄性收集的。用BF5F扩展剂（8%甘油）稀释，用0.5 ml吸管在干冰上冷冻，液氮中保存的精液解冻后精子活力为60-70%。将阴道内黄体酮释放装置（cidr - G型）置入20只成年鹿尾体内14 d。在取出CIDR装置70小时后，通过腹腔镜将精液（每个子宫角7.5-10 × 10(6)个活动精子）沉淀。通过分析每周收集3至7次的空尿中的妊娠二醇-3 α -葡糖苷酸（PdG）浓度，监测人工智能前后卵巢活动。在CIDR装置插入期间，所有小鼠的尿PdG分布等于或高于正常黄体相浓度。在取出装置的48小时内，20只雌性中有17只的PdG浓度恢复到基线值，12只在此时发生了行为性发情。在尿PdG持续升高的基础上，20只母鼠中有9只在AI后90天被诊断为怀孕，所有母鼠在平均妊娠241.1天（范围，235-245）后分娩后代。7只单胎（2只雌性，5只雄性）活产并存活，1只单胎和1对双胞胎（3只雌性）死产。（摘要删节250字）
This study tested the efficacy of assisted reproduction (synchronization of oestrus and intrauterine artificial insemination (AI)) in contributing to the captive propagation of an endangered species, the Eld's deer (Cervus eldi thamin). Semen was collected from males preselected on the basis of under-represented genotype. Motility of spermatozoa after thawing from ejaculates diluted with BF5F extender (8% glycerol), frozen on dry ice in 0.5 ml straws and stored in liquid nitrogen was 60-70%. Intravaginal progesterone-releasing devices (controlled internal drug release, CIDR-type G) were inserted into 20 adult Eld's deer hinds for 14 days. In all hinds, semen (7.5-10 x 10(6) motile spermatozoa per uterine horn) was deposited by laparoscopy performed 70 h after removal of the CIDR device. Ovarian activity, before and after AI, was monitored by analysing pregnanediol-3 alpha-glucuronide (PdG) concentrations in voided urine collected three to seven times per week. During the period of CIDR device insertion, urinary PdG profiles were equal to, or above, normal luteal phase concentrations in all hinds. Within 48 h of device withdrawal, PdG concentrations returned to baseline values in 17 of the 20 females, and the onset of behavioural oestrus occurred at this time in 12 hinds. On the basis of sustained increases in urinary PdG, 9 of the 20 hinds were diagnosed as pregnant by 90 days after AI, all of which delivered offspring after a mean gestation of 241.1 days (range, 235-245). Seven singletons (two females, five males) were born alive and survived, and one singleton and one set of twins were stillborn (three females).(ABSTRACT TRUNCATED AT 250 WORDS)