Metabolomic Study to Determine the Mechanism Underlying the Effects of Sagittaria sagittifolia Polysaccharide on Isoniazid- and Rifampicin-Induced Hepatotoxicity in Mice
Metabolomic Study to Determine the Mechanism Underlying the Effects of Sagittaria sagittifolia Polysaccharide on Isoniazid- and Rifampicin-Induced Hepatotoxicity in Mice
复制标题
代谢组学研究确定慈姑多糖对异烟肼和利福平诱导的小鼠肝毒性作用的机制。
DOI：
10.3390/molecules23123087
复制
发表时间：
2018-12-01
期刊：
影响因子：
4.6
通讯作者：
Liao, Yan
中科院分区：
文献类型：
--
作者：
Ke, Xiu-Hui;Wang, Chun-Guo;Liao, Yan
本研究采用基于超高效液相色谱-高分辨率质谱（UPLC-HRMS）的非靶向代谢谱分析方法，表征了与矢状叶多糖（SSP）对异烟肼（INH）和利福平（RFP）诱导的小鼠肝毒性保护作用相关的血浆代谢谱。从ssp处理小鼠的血浆中鉴定出14种潜在的生物标志物。通过对相关代谢途径的研究，进一步阐明了SSP对INH和RFP联合引起的肝毒性的保护作用（INH/RFP）。发现INH/RFP破坏脂肪酸代谢、三羧酸循环、氨基酸代谢、牛磺酸代谢和鸟氨酸循环。代谢组学研究结果表明，SSP通过部分调节紊乱的代谢途径，对INH/ rfp诱导的肝损伤具有保护作用。
In this study, a non-targeted metabolic profiling method based on ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) was used to characterize the plasma metabolic profile associated with the protective effects of the Sagittaria sagittifolia polysaccharide (SSP) on isoniazid (INH)and rifampicin (RFP)-induced hepatotoxicity in mice. Fourteen potential biomarkers were identified from the plasma of SSP-treated mice. The protective effects of SSP on hepatotoxicity caused by the combination of INH and RFP (INH/RFP) were further elucidated by investigating the related metabolic pathways. INH/RFP was found to disrupt fatty acid metabolism, the tricarboxylic acid cycle, amino acid metabolism, taurine metabolism, and the ornithine cycle. The results of the metabolomics study showed that SSP provided protective effects against INH/RFP-induced liver injury by partially regulating perturbed metabolic pathways.