Photoelectrochemical Aptasensors Constructed with Photosensitive PbS Quantum Dots/TiO2 Nanoparticles for Detection of Kanamycin.
Photoelectrochemical Aptasensors Constructed with Photosensitive PbS Quantum Dots/TiO2 Nanoparticles for Detection of Kanamycin.
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用光敏 PbS 量子点/TiO2 纳米粒子构建的光电化学适体传感器用于检测卡那霉素。
DOI：
10.1021/acs.langmuir.0c03593
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Zhigang Jin
中科院分区：
文献类型：
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作者：
Yichen Xing;Xiaoxiao Chen;Boxing Jin;Piaopiao Chen;Chaobiao Huang;Zhigang Jin
卡那霉素是一种广泛使用的兽药，其滥用对人类健康造成严重威胁，迫切需要对动物源性食品中卡那霉素残留量进行高特异性、高灵敏度的检测。在这里，我们开发了一种光电化学（PEC）生物传感器，用于快速定量的假名，与硫化铅量子点/二氧化钛纳米粒子（PbS量子点/TiO 2纳米粒子）作为光敏复合材料，假名特异性的DNA适体作为功能传感器，和钌（III）六胺（Ru（NH3）63+）作为信号助推器。为了制备PEC适体传感器，分别用TiO 2纳米粒子、PbS量子点和聚乙烯亚胺（PEI）修饰氧化铟锡电极，然后通过戊二醛将端氨基适体探针连接到PEI上。最后，Ru（NH3）63+附着在适配体的表面上以增加光电流强度。当Kana与Ru（NH3）63+竞争性结合到固定在适体传感器表面上的适体时，Ru（NH3）63+将从适体释放，导致光电流信号降低。在1.0-300.0 nmol L-1范围内，光电流位移与Kana浓度的对数呈良好的线性关系，检测限为0.161 nmol L-1。重要的是，由于与卡纳的特异性相互作用，PEC适体传感器呈现出良好的检测选择性，并成功地实现了蜂蜜和牛奶中卡纳的定量，这表明PEC适体传感器具有快速检测动物源性食品中残留卡纳的潜力。
Kanamycin (Kana) is widely used as a veterinary medicine and its abuse causes a serious threat to human health, raising the urgent demand for detection of residual Kana in animal-derived food with high specificity and sensitivity. Here, we developed a photoelectrochemical (PEC) biosensor for rapid quantification of Kana, with lead sulfide quantum dots/titanium dioxide nanoparticles (PbS QDs/TiO2 NPs) as a photosensitive composite, a Kana-specific DNA aptamer as a functional sensor, and ruthenium(III) hexaammine (Ru(NH3)63+) as a signal booster. To prepare the PEC aptasensor, TiO2 NPs, PbS QDs, and polyethyleneimine (PEI) were respectively used to modify the indium tin oxide electrode, and then the amine-terminated aptamer probe was connected to the PEI via glutaraldehyde. Finally, Ru(NH3)63+ was attached on the surface of the aptamer to increase the photocurrent intensity. When Kana binds competitively with Ru(NH3)63+ to the aptamer immobilized on the surface of the aptasensor, Ru(NH3)63+ will be released from the aptamer, resulting in a decrease of the photocurrent signal. This PEC aptasensor exhibits a good linear relationship between the photocurrent shift and the logarithm of Kana concentration within the range of 1.0-300.0 nmol L-1, and the detection limit is 0.161 nmol L-1. Importantly, the PEC aptasensor presented good detection selectivity owing to specific interaction with Kana and was successfully implemented to quantify Kana in honey and milk, suggesting that the PEC aptasensor has the potential of rapid detection of residual Kana in animal-derived foods.