Discrimination of intracellular calcium store subcompartments using TRPV1 (transient receptor potential channel, vanilloid subfamily member 1) release channel activity.
Discrimination of intracellular calcium store subcompartments using TRPV1 (transient receptor potential channel, vanilloid subfamily member 1) release channel activity.
复制标题
使用 TRPV1（瞬时受体电位通道，香草酸亚家族成员 1）释放通道活性区分细胞内钙储存亚区室。
DOI：
10.1042/bj20021381
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Penner,Reinhold
中科院分区：
文献类型：
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作者：
Turner,Helen;Fleig,Andrea;Stokes,Alexander;Kinet,Jean-Pierre;Penner,Reinhold
钙库操纵的钙释放激活钙电流（ICRAC）是钙进入非兴奋性细胞的主要机制。ICRAC 补充钙库并允许持续的钙信号传导。含肌醇 1,4,5-三磷酸受体 (InsP3R) 的储存与 ICRAC 之间的关系尚不清楚。全局 InsP3R 储存耗尽与 ICRAC 激活耦合的模型可能过于简单化，因为细胞内储存的释放和再填充活动是异质的。在这里，我们使用配体门控钙通道 TRPV1（瞬时受体电位通道，香草酸亚家族成员 1）作为一种新工具来探测肥大细胞系中的存储异质性并定义细胞内钙区室。 TRPV1具有作为细胞内释放通道的活性，但不介导整体钙储存耗尽，并且不侵入与ICRAC结合的钙储存。细胞内 TRPV1 定位于含有 InsP3R 库的子集。 TRPV1 敏感性在功能上细分了 InsP3 敏感存储，负责存储补充的肌浆/内质网 Ca2+-ATP 酶亚型的异质性也是如此。这些结果提供了明确的证据，证明 InsP3 可释放的钙储存内存在特定的“CRAC 储存”，并描述了一种操纵细胞内游离钙的新方法。
The store-operated calcium-release-activated calcium current,ICRAC, is a major mechanism for calcium entry into non-excitable cells.ICRACrefills calcium stores and permits sustained calcium signalling. The relationship between inositol 1,4,5-trisphosphate receptor (InsP3R)-containing stores andICRACis not understood. A model of global InsP3R store depletion coupling withICRACactivation may be simplistic, since intracellular stores are heterogeneous in their release and refilling activities. Here we use a ligand-gated calcium channel, TRPV1 (transient receptor potential channel, vanilloid subfamily member 1), as a new tool to probe store heterogeneity and define intracellular calcium compartments in a mast cell line. TRPV1 has activity as an intracellular release channel but does not mediate global calcium store depletion and does not invade a store coupled withICRAC. Intracellular TRPV1 localizes to a subset of the InsP3R-containing stores. TRPV1 sensitivity functionally subdivides the InsP3-sensitive store, as does heterogeneity in the sarcoplasmic/endoplasmic-reticulum Ca2+-ATPase isoforms responsible for store refilling. These results provide unequivocal evidence that a specific ‘CRAC store’ exists within the InsP3-releasable calcium stores and describe a novel methodology for manipulation of intracellular free calcium.