Adsorptive stripping voltammetric detection of daunomycin at a bismuth bulk electrode
Adsorptive stripping voltammetric detection of daunomycin at a bismuth bulk electrode
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DOI：
10.1002/elan.200403178
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发表时间：
2005-03-01
期刊：
影响因子：
3
通讯作者：
Flechsig, GU
中科院分区：
文献类型：
--
作者：
Bucková, M;Gründler, P;Flechsig, GU
铋电极表现出与最常用的有毒汞电极相似的电化学性质，并且代表了用于在负电位下检测电活性化合物的合适、可靠且环境友好的工具。铋电极在-200 ~-1400 mV（vs. SCE）范围内具有较宽的工作电位窗口，可用于药物柔红霉素在nmol/L浓度范围内的吸附溶出伏安法测定。与金电极相比，道诺霉素在铋电极上的电压信号更高且形状非常好。富集电位为-0.65 V（vs. SCE），富集时间为2 ~ 10 min是道诺霉素在铋电极表面富集的最佳条件。在100 - 1000 nmol/L浓度范围内（沉积2 min）和10 - 100 nmol/L浓度范围内（沉积5 min），吸附溶出响应呈线性，检测限为5 nmol/L（沉积8 min）。重复性的特征为相对标准偏差7.1%（10次测量）。在两次连续测量之间，必须通过在湿纸上抛光或在96%乙醇中搅拌5分钟来再生电极。
The bismuth electrode exhibits similar electrochemical properties as most commonly used toxic mercury electrode and represents a suitable, reliable and also environmentally friendly tool for the detection of electroactive compounds at negative potentials. A wide operational potential window of the bismuth electrode from - 200 mV to - 1400 mV (vs. SCE) was found. Bismuth bulk electrodes (BiBE) are very convenient for sensitive adsorptive stripping voltammetric (AdSV) determination of the drug daunomycin in the nmol/L concentration range. In comparison with gold electrodes, the voltarnmetric signals of daunomycin at the bismuth electrode are higher and very well shaped. An accumulation potential of - 0.65 V (vs. SCE) and accumulation time from 2 to 10 min were found to be optimal conditions for the preconcentration of daunomycin on the bismuth electrode surface. The adsorptive stripping response is linear over '100 - 1000 nmol/L concentration range (for 2 min deposition) and over 10 - 100 nmol/L concentration range (for 5 min deposition) with a detection limit of 5 nmol/L (for 8 min deposition). Repeatability was characterized by relative standard deviation 7.1% (for 10 measurements). The electrode must be regenerated between two consecutive measurements by polishing on a damp paper or by stirring in 96% ethanol for 5 min.