Developmental changes in the bark lectin of Sophora japonica L.
Developmental changes in the bark lectin of Sophora japonica L.
复制标题
国槐树皮凝集素的发育变化。
作者：
K. Baba;M. Ogawa;A. Nagano;H. Kuroda;K. Sumiya
凝集素是S.日本的。用抗种子凝集素抗体进行免疫组化，结果表明凝集素定位于射线和轴向薄壁组织。在形成层、筛管和伴胞中均未观察到凝集素或其它交叉反应物质。分布和定位的变化与组织的发展。树皮中凝集素的含量在一年中变化很大，夏季的平均值约为冬季的50%。冬季树皮中凝集素的分布从最内层（最年轻）到最外层（最老）区域相似。相反，在夏季，最里面的区域几乎不包含任何凝集素，最外面的区域包含的凝集素比中间少。凝集素在组织和细胞中的定位也根据组织年龄而不同。在当年产生的新组织中，lectip在夏季不存在，在秋季位于细胞壁和液泡之间的细胞质层中，在冬季被隔离在液泡中。另一方面，旧组织（前一年形成）中的凝集素全年主要位于液泡中，秋季仅在细胞质层中有非常少的含量。在最外层（最古老的）地区，其中凝集素含量低，在夏季，细胞接壤的外部树皮从来没有包含任何凝集素在夏季。在秋季的新组织的细胞内定位，由免疫金电子显微镜确定，在内质网和囊泡的内腔，与金颗粒几乎不存在于细胞质中。从这些研究结果中，我们得出结论，凝集素合成的内质网和最有力的新组织在秋季，它主要是消耗在最外层的树皮地区，扩张发生和或软木形成层分化。
Lectin is the major protein in the phloem tissue of S. japonica. By immunohistochemistry using anti-seed lectin antibody it was demonstrated that the lectin was localized in the ray and the axial parenchyma. Neither lectin nor other cross-reactive materials were observed in the cambium, sieve tubes and companion cells. The distribution and localization changed in relation to tissue development. Lectin content in the bark changed during the year, the average in summer being about 50% of that in winter. The distribution of lectin in the bark in winter was similar from the innermost (youngest) to the outermost (oldest) region. In contrast, in summer the innermost region hardly contained any lectin, and the outermost region contained less lectin than the middle. Lectin localization in tissues and cells differed also depending on tissue age. In new tissue, produced in the current year, lectip was absent in summer, was located in the cytoplasmic layer between cell wall and vacuole in autumn, and sequestered in the vacuoles in winter. On the other hand, lectin in old tissue (formed in the previous year) was located throughout the year mainly within the vacuoles, with only very small contents in the cytoplasmic layer in autumn. Within the outermost (oldest) region, in which the lectin content was low in summer, the cells which bordered the outer bark never contained any lectin in summer. The intracellular localization in autumn in new tissue, determined by immunogold electron microscopy, was in the lumen of the endoplasmic reticulum and vesicles, with gold particles hardly present in the cytoplasm. From these findings we conclude that lectin is synthesized on the endoplasmic reticulum and most vigorously in the new tissue in autumn, and that it is mainly consumed in the outermost bark regions, where dilatation occurs and-or where cork cambium is differentiated.
影响因子：
2.9
作者：
BLAKE, MS;JOHNSTON, KH;GOTSCHLICH, EC
通讯作者：
GOTSCHLICH, EC