Protein Crystallography and Site-Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis
Protein Crystallography and Site-Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis
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坂井 Ideonella sakaiensis 聚对苯二甲酸乙二醇酯水解酶 PETase 的蛋白质晶体学和定点突变分析
DOI：
10.1002/cbic.201800097
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发表时间：
2018-07-16
期刊：
影响因子：
3.2
通讯作者：
Bao, Rui
中科院分区：
文献类型：
--
作者：
Liu, Bing;He, Lihui;Bao, Rui
与传统的回收策略不同，生物降解是处理聚对苯二甲酸乙二醇酯（PET）废物的可持续解决方案。PETase是一种新发现的酶，对PET具有高效和特异性，因此是PET降解的主要候选酶。在生化分析的基础上，我们提出，一个广泛的底物结合口袋是至关重要的，其优异的能力，水解结晶PET。结构指导的定点突变揭示了PETase催化效率的改善，为PETase的分子工程如何优化其在生物催化中的应用提供了有价值的见解。
Unlike traditional recycling strategies, biodegradation is a sustainable solution for disposing of poly(ethylene terephthalate) (PET) waste. PETase, a newly identified enzyme from Ideonella sakaiensis, has high efficiency and specificity towards PET and is, thus, a prominent candidate for PET degradation. On the basis of biochemical analysis, we propose that a wide substrate-binding pocket is critical for its excellent ability to hydrolyze crystallized PET. Structure-guided site-directed mutagenesis revealed an improvement in PETase catalytic efficiency, providing valuable insight into how the molecular engineering of PETase can optimize its application in biocatalysis.