Differential mesengenic potential and expression of stem cell-fate modulators in mesenchymal stromal cells from human-term placenta and bone marrow
Differential mesengenic potential and expression of stem cell-fate modulators in mesenchymal stromal cells from human-term placenta and bone marrow
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DOI：
10.1002/jcp.24014
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发表时间：
2012-09-01
影响因子：
5.6
通讯作者：
Cooper-White, Justin J.
中科院分区：
文献类型：
--
作者：
Jaramillo-Ferrada, Pamela A.;Wolvetang, Ernst J.;Cooper-White, Justin J.
在过去的十年中，胎盘作为再生医学的干细胞来源引起了越来越多的关注。特别是，羊膜上有大量间充质基质细胞(MSCs)。在本研究中，我们对体外扩增的人羊膜间充质干细胞(HAMSCs)和人足月胎盘绒毛膜(HCMSCs)和成人骨髓(HBMSCs)进行了鉴定。结果表明，hAMSCs、hCMSCs和hBMSCs表达典型的间充质(CD73、CD90、CD105、CD44、CD146、CD166)和多能(Oct-4、Sox2、Nanog、Lin28和KLF4)标志，但不表达造血标志(CD45、CD34)。体外扩增的hAMSCs被发现来自胎儿，而hCMSCs培养只含有母体细胞。HCMSCs的细胞增殖率明显高于hAMSCs和hBMSCs。整合素图谱显示，在三种细胞来源之间，a亚单位的表达存在显著差异。而N-CAM(CD56)仅在hAMSCs和hCMSCs中表达，而在hBMSCs中不表达。当被诱导分化时，hAMSCs和hCMSCs显示出很强的软骨和成骨分化潜能，但成脂转化能力非常有限。相反，hBMSCs在三个谱系上表现出很强的分化潜能。这些结果说明了来自不同本体来源的MSCs如何表现出不同的细胞命运调节因子表达和中胚层分化能力。J.细胞。物理。2012年，227：32343242。(C)2011年威利期刊公司。
Placenta has attracted increasing attention over the past decade as a stem cell source for regenerative medicine. In particular, the amniochorionic membrane has been shown to harbor populations of mesenchymal stromal cells (MSCs). In this study, we have characterized ex vivo expanded MSCs from the human amniotic (hAMSCs) and chorionic (hCMSCs) membranes of human full-term placentas and adult bone marrow (hBMSCs). Our results show that hAMSCs, hCMSCs, and hBMSCs express typical mesenchymal (CD73, CD90, CD105, CD44, CD146, CD166) and pluripotent (Oct-4, Sox2, Nanog, Lin28, and Klf4) markers but not hematopoietic markers (CD45, CD34). Ex vivo expanded hAMSCs were found to be of fetal origin, while hCMSCs cultures contained only maternal cells. Cell proliferation was significantly higher in hCMSCs, compared to hAMSCs and hBMSCs. Integrin profiling revealed marked differences in the expression of a subunits between the three cell sources. Cadherin receptors were consistently expressed on a subset of progenitors (ranging from 1% to 60%), while N-CAM (CD56) was only expressed in hAMSCs and hCMSCs but not in hBMSCs. When induced to differentiate, hAMSCs and hCMSCs displayed strong chondrogenic and osteogenic differentiation potential but very limited capacity for adipogenic conversion. In contrast, hBMSCs showed strong differentiation potential along the three lineages. These results illustrate how MSCs from different ontological sources display differential expression of cell-fate mediators and mesodermal differentiation capacity. J. Cell. Physiol. 227: 32343242, 2012. (C) 2011 Wiley Periodicals, Inc.