IDENTIFICATION OF A HUMAN SRC HOMOLOGY 2-CONTAINING PROTEIN-TYROSINE-PHOSPHATASE - A PUTATIVE HOMOLOG OF DROSOPHILA CORKSCREW
IDENTIFICATION OF A HUMAN SRC HOMOLOGY 2-CONTAINING PROTEIN-TYROSINE-PHOSPHATASE - A PUTATIVE HOMOLOG OF DROSOPHILA CORKSCREW
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DOI：
10.1073/pnas.89.23.11239
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发表时间：
1992-12-01
影响因子：
11.1
通讯作者：
NEEL, BG
中科院分区：
文献类型：
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作者：
FREEMAN, RM;PLUTZKY, J;NEEL, BG
src 同源 2 (SH2) 结构域直接结合特定的磷酸酪氨酰蛋白。最近，鉴定出含有 SH2 的蛋白酪氨酸磷酸酶 (PTP)。使用简并寡核苷酸和 PCR，我们克隆了另一个 PTP SH-PTP2 的 cDNA，它包含两个 SH2 结构域并普遍表达。当在大肠杆菌中表达时，SH-PTP2 表现出酪氨酸特异性磷酸酶活性。 SH-PTP2 与果蝇基因开瓶器 (csw) 之间的高度相似性及其相似的表达模式表明 SH-PTP2 是人类开瓶器同源物。 SH-PTP2、SH-PTP1、开瓶器和其他含有 SH2 的蛋白质之间的序列比较表明存在特定于 PTP 的 SH2 结构域亚家族，而含有 SH2 的 PTP 的 PTP 结构域与其他酪氨酸磷酸酶的比较表明存在含有 SH2 结构域的 PTP 亚家族。由于开瓶器是末端类信号转导途径的成员，它与 D-raf 协同作用，积极转导受体酪氨酸激酶躯干产生的信号，这些发现表明 SH-PTP2 可能参与哺乳动物信号转导的几种机制。
src homology 2 (SH2) domains direct binding to specific phosphotyrosyl proteins. Recently, SH2-containing protein-tyrosine-phosphatases (PTPs) were identified. Using degenerate oligonucleotides and the PCR, we have cloned a cDNA for an additional PTP, SH-PTP2, which contains two SH2 domains and is expressed ubiquitously. When expressed in Escherichia coli, SH-PTP2 displays tyrosine-specific phosphatase activity. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (csw) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog. Sequence comparisons between SH-PTP2, SH-PTP1, corkscrew, and other SH2-containing proteins suggest the existence of a subfamily of SH2 domains found specifically in PTPs, whereas comparison of the PTP domains of the SH2-containing PTPs with other tyrosine phosphatases suggests the existence of a subfamily of PTPs containing SH2 domains. Since corkscrew, a member of the terminal class signal transduction pathway, acts in concert with D-raf to positively transduce the signal generated by the receptor tyrosine kinase torso, these findings suggest several mechanisms by which SH-PTP2 may participate in mammalian signal transduction.