Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle.
Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle.
复制标题
将 FACS 分离的肌肉干细胞植入受伤的骨骼肌中。
DOI:
10.1007/978-1-4939-6771-1_11
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发表时间:
2017
期刊:
影响因子:
--
通讯作者:
Sacco,Alessandra
中科院分区:
文献类型:
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作者:
Tierney,Matthew;Sacco,Alessandra
Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.