Hand-held bioprinting for de novo vascular formation applicable to dental pulp regeneration
Hand-held bioprinting for de novo vascular formation applicable to dental pulp regeneration
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DOI：
10.1080/03008207.2019.1640217
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发表时间：
2019-07-11
影响因子：
2.9
通讯作者：
Esteves-Oliveira, Marcella
中科院分区：
文献类型：
--
作者：
Campos, Daniela F. Duarte;Zhang, Siyuan;Esteves-Oliveira, Marcella
研究目的：深龋病变可能会导致不可逆的牙髓炎，目前的牙髓治疗通常会去除整个牙髓组织，这最终会缩短牙齿的寿命。目前，最常见的治疗方法是去除感染组织，用惰性合成材料填充根管。组织工程方法是目前治疗的重要替代方案，因为它们可以潜在地保持牙齿的生物功能，而不是牺牲it.Materials和方法：在这项研究中，我们提出了一种基于手持原位生物打印策略的组织工程方法。我们的方法能够生物打印具有合适的流变学，结构和生物学特性的细胞负载胶原蛋白生物墨水，这使得根管中的血管发生成为可能。结果如下：通过振荡振幅扫描测试测量生物可打印的生物墨水的流变特性，并通过体外培养后的宏观评价证实，其中打印的生物墨水保持其原始形式而不收缩。此外，我们显示了在生物可打印的生物墨水中成功血管发生的证据，其具有可比的质量和数量以控制纤维蛋白和胶原蛋白非生物可打印的水凝胶。结论：我们的结论是，手持生物打印具有原位治疗牙科疾病的潜力，成功地证明了血管形成，作为维持牙齿生物功能的资产。
Aim of the study: Deep carious lesions may cause irreversible pulpitis and the current endodontic treatment typically removes the whole dental pulp tissue, which finally reduces lifespan of the teeth. Nowadays, the most frequent treatment is based on removing the infected tissue and filling the root canal with inert synthetic materials. Tissue engineering approaches are important alternatives to the current treatment, because they can potentially maintain the biological function of the tooth instead of sacrificing it. Materials and Methods: In this study, we propose a tissue engineering approach based on a hand-held in situ bioprinting strategy. Our approach enabled bioprinting of cell-loaded collagen-based bioinks with suitable rheological, structural and biological properties, which allowed for vasculogenesis in the root canal. Results: The rheological properties of the bioprintable bioink were measured by oscillatory amplitude sweep testing and were corroborated by macroscopic evaluation after in vitro culture, in which printed bioinks maintained their original form without contraction. Moreover, we showed evidence for successful vasculogenesis in bioprintable bioinks with comparable quality and quantity to control fibrin and collagen non-bioprintable hydrogels. Conclusions: We conclude that hand-held bioprinting holds potential for in situ treatment of dental diseases with successful evidence for vascular tube formation, as an asset for maintenance of the biological function of the tooth.