CHONDRONS EXTRACTED FROM CANINE TIBIAL CARTILAGE - PRELIMINARY-REPORT ON THEIR ISOLATION AND STRUCTURE
CHONDRONS EXTRACTED FROM CANINE TIBIAL CARTILAGE - PRELIMINARY-REPORT ON THEIR ISOLATION AND STRUCTURE
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DOI：
10.1002/jor.1100060312
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发表时间：
1988-05-01
影响因子：
2.8
通讯作者：
BEAUMONT, BW
中科院分区：
文献类型：
--
作者：
POOLE, CA;FLINT, MH;BEAUMONT, BW
我们报道了从新鲜和固定的犬胫骨软骨匀浆标本中分离出的单个和多个软骨的形态和结构。位相对比、Nomarski和扫描电子显微镜观察显示，每个软骨由一个软骨细胞和它的细胞周围基质组成，包裹在一个“毛毡状”的细胞周囊内。从软骨匀浆中提取完整的软骨证实了Condron概念的结构有效性，并强调了胶囊的内在机械强度。磨损的胶原纤维从多个软骨骨柱放射出来，表明包膜成分和II型胶原纤维之间存在抗剪切的结构相互关系。未来软骨提取技术的发展可能为研究关节软骨细胞及其细胞周围微环境的结构、生化和功能提供独特的模型。
We report on the morphology and structure of single and multiple chondrons isolated from homogenized samples of fresh and fixed canine tibial cartilage. Phase contrast, Nomarski, and scanning electron microscopy observations show each chondron to be composed of a chondrocyte and its pericellular matrix enclosed within a "felt-like" pericellular capsule. The extraction of intact chondrons from cartilage homogenates confirms the structural validity of the condron concept and emphasizes the intrinsic mechanical strength of the capsule. Frayed collagen fibers radiate from multiple chondron columns suggesting a shear-resistant, structural interrelationship between capsular components and type II collagen fibers. Future development of chondron extraction procedures could provide a unique model with which to study the structure, biochemistry, and function of articular cartilage chondrocytes and their pericellular microenvironment.