The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model.
The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model.
复制标题
葡萄球菌胡萝卜素生成在实验性心内膜炎模型中对宿主防御肽的抵抗和体内毒力中的作用。
DOI：
10.1093/femspd/ftv056
复制
发表时间：
2015
影响因子：
3.3
通讯作者：
Mishra,NagendraN
中科院分区：
文献类型：
--
作者：
Xiong,YanQ;Yang,Soo-Jin;Tong,StevenYC;Alvarez,DanyaN;Mishra,NagendraN
新描述的物种，银色葡萄球菌，与其姐妹物种，S。aureusandS. schweitzeri的葡萄球菌缺乏类胡萝卜素色素葡萄黄素的产生，而银色葡萄球菌缺乏负责的遗传位点OPQMN。argenteusstrains，MSHR 1132和SCC 1165。与含有类胡萝卜素操纵子的质粒（pTX-crtOPQMN）互补后，与野生型相比，两种互补菌株均显示出大量的类胡萝卜素产生，从而增加了细胞膜的刚性。令人惊讶的是，两种补充OPQMN的菌株在体外对宿主防御肽LL-37和hNP-1的敏感性增加，而在实验性兔心内膜炎模型中的毒力降低。
The defining hallmark of the newly described species,Staphylococcus argenteus, in comparison to its sister species,S. aureusandS. schweitzeri, is the absence of production of the carotenoid pigment, staphyloxanthin.Staphylococcus argenteuslacks the responsible genetic locuscrtOPQMN.We examined the impact of carotenoid synthesis in two non-pigmentedS. argenteusstrains, MSHR1132 and SCC1165. Following complementation with a plasmid containing the carotenoid operon (pTX-crtOPQMN), compared to wild type, both complemented strains showed substantial carotenoid production, with a resultant increase in cell membrane rigidity. Surprisingly, bothcrtOPQMN-complemented strains exhibited increased susceptibility to the host defense peptides, LL-37 and hNP-1in vitro, and reduced virulence in an experimental rabbit endocarditis model.