Control of gene expression in neurons by the use of in vivo electroporation and the tetracycline system
Control of gene expression in neurons by the use of in vivo electroporation and the tetracycline system
复制标题
使用体内电穿孔和四环素系统控制神经元中的基因表达
DOI：
10.1007/978-1-4939-2459-2_15
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Tetsuichiro Saito
中科院分区：
文献类型：
--
作者：
Tatsuya Sato;Yuko Muroyama;Tetsuichiro Saito
体内电穿孔是一种将外源基因导入神经干/祖细胞的有效技术。基因功能已在转染的神经干/祖细胞及其后代，分化的神经元进行了分析。在神经元中工作的诱导型基因表达系统是期望的，以检测基因功能，特别是在神经元中。我们最近表明，基因诱导是严格控制在有丝分裂后的神经元在小鼠胚胎和出生后的阶段，使用体内电穿孔和最新版本的四环素（泰特）的系统。在这里，我们描述的方法，小鼠在体内电穿孔结合Tet-On系统精确时空控制基因表达。
In vivo electroporation is a powerful technique to transfect foreign genes into neural stem/progenitor cells in the developing nervous system. Gene function has been analyzed in transfected neural stem/progenitor cells and their descendants, differentiated neurons. An inducible gene expression system working in neurons is desirable to examine gene function specifically in neurons. We have recently shown that gene induction is strictly controlled in postmitotic neurons in mice at both embryonic and postnatal stages using in vivo electroporation and the newest version of the tetracycline (Tet)-On system. Here we describe the method of mouse in vivo electroporation combined with the Tet-On system for precise spatiotemporal control of gene expression.